Penambahan Ekstrak Alga Sargassum duplicatum Bory pada Medium Kultur In Vitro terhadap Pertumbuhan seedling Anggrek Vanda tricolor Lindl.

Eko Adiguna (1) , Murni Dwiati (2) , Kamsinah Kamsinah (3)


(1) Fakultas Biologi Universitas Jenderal Soedirman Purwokerto
(2) Fakultas Biologi Universitas Jenderal Soedirman Purwokerto
(3) Fakultas Biologi Universitas Jenderal Soedirman Purwokerto


Abstrak


Vanda tricolor is an orchid species that has many advantages, some of which are due to its beautiful and various flowers of unique shape and size. Propagation of V. tricolor can be performed by in vitro culture.In vitro culture technique needs appropriate medium to support orchid growth. An algae species, Sargassum duplicatum, can be added into the medium, since it contains growth regulating substances, such as auxin and gibberellin, which are useful for seedling growth. This study aims to know the effect of S. duplicatum application into in vitro culture medium of V. tricolor on the seedling growth and to know the best application of the algaein increasing seedling growth. An experimental method was arranged in a Completely Randomized Design (CRD). The treatments were S. duplicatum weight of 0, 12, 24, 36, 48 and 60 g/L replicated three times respectively. The variable examined was seedling growth with parameters comprising day of new leaf emergence, leaf number, leaf length, day of new root emergence, root number, root length and plant height. Data were analyzed using ANOVA or F test with confidence level of 95% and 99%. LSD was carried out when F test showed significant difference. The results reveal that application of S. duplicatum extract has significant effect on the growth of V. tricolor seedling. The best application is of 36 g/L algae weight.

Kata Kunci


Sargassum duplicatum, seedling, Vanda tricolor

Teks Lengkap:

PDF

Referensi


Anggadiredja, J., Zatnika, A., Sujatmiko, W., Ismail, S., Moor, Z Aryanti, W.S. & Izzati, M., 1993. Teknologi Produk Perikanan dalam Industri Farmasi; Potensi dan Pemanfaatan Makro Alga Laut. Skripsi. Bogor: Fakultas Perikanan Institut Pertanian Bogor.

Basmal, J., 2009. Prospek Pemanfaatan Rumput Laut Sebagai Bahan Pupuk Organik. J.Skualen, 4(1): 1-8.

Basmal, J., Kusumawati, K., Utomo, B.S.B. 2015. Mutu Sap Liquid Rumput Laut Sargassum yang Diekstrak Menggunakan Kalium Hidroksida sebagai Bahan Pupuk. JPB Kelautan dan Perikanan 10(2): 143-153.

Bety, Y.A., 2004. Medium Sapih Alternatif untuk Plantlet Anggrek Vanda. Journal Horticultura, (14): 1-2.

Bey, Y., Syafii, W. & Ngatifah, N., 2006. Pengaruh Pemberian Giberelin pada medium Vacin & Went terhadap perkecambahan Biji Anggrek Bulan (Phalaenopsis amabilis BL) secara In Vitro. Jurnal Biogenesis, 14(1): 15-21.

Cai, X., Feng, Z., Xu, W., Hou, B., & Ding, X., 2011. Genetic Diversity And Population Structure of An Endangered Orchid (Dendrobium loddigesii Rolfe) from China Revealed by SRAP Markers. Scientia Horticulturae, 129(1): 877 – 881.

Douglas, J.S., 1985. Advanced Guide to hydrophonic (Soilless Cultivation). London: Pelham Books Ltd.

Falasifa, A., Slameto & Hariyono, K., 2014. Pengaruh Pemberian Ekstrak Ascophyllum nodosum Serbuk dan Cair terhadap Pertumbuhan Tanaman Selada Berdaun Merah (Lactuca sativa var. Crispa). Berkala Ilmiah Pertanian, 3(1): 62-24.

Handayani, T., Sutarno & Setyawan, A.D., 2014. Analisis Komposisi Hara Rumput Laut Sargassum crassifolium J. Agardh. Biofarmasi, 2: 45-52.

Iswanto, H., 2002. Petunjuk Perawatan Anggrek. Jakarta: PT. Agro Medium Pustaka.

Kalaivanan, C. & Venkatesalu, V., 2012. Utilization of Seaweed Sargassum myriocystum Extracts as A Stimulant of Seedlings of Vigna Mungo (L.) Hepper. Span. J. Agric. Res., (10): 466-70.

Mahfut & Daryono, B.S., 2014. Deteksi Odontoglossum Ringspot Virus terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul. Jurnal Ilmiah Biologi, 2(2): 101-08.

Sahtiana, N., 2016. Penggunaan Pupuk Organik dan Ekstrak Kersen (Muntingia calabura L.) untuk Substitusi Medium VW (Vacint And Went) dan Sukrosa pada Medium Subkultur Anggrek Vanda tricolor secara In Vitro. Skripsi. Yogyakarta: UMY.

Salisbury, F.B. & Ross, C.W., 1995. Fisiologi Tumbuhan I. Bandung: Institut Teknologi Bandung.

Thirumaran, G., Arumugam, M., Arumugam, R. & Anantharaman, P., 2009. Effect of Seaweed Liquid Fertilizer on Growth and Pigment Concentration of Abelmoschus esculentus (L) Medikus. Am-Euras. J. Agro, II(2): 57-66.

Widiastoety, D., 2003. Menghasilkan Anggrek Silangan. Jakarta: Penebar Swadaya.

Widyastoety, D. & Santi, A., 2012. Keunggulan Kelompok Anggrek Vanda dalam Meningkatkan Variasi dan Kualitas Anggrek Bunga Potong. In Prosiding Seminar Nasional Anggrek 2012. Cianjur, 2012. Balai Penelitian Tanaman Hias.

Zaki, I.F., 2014. Kajian Pengaruh Pemberian Sitokinin Terhadap Pertumbuhan Tanaman. In Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan. Surabaya, 2014. Direktorat Jenderal Perkebunan.


Article Reads

Total: 525 Abstrak: 310 PDF: 213

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • Saat ini tidak ada refbacks.


 

This website is maintained by:
The Faculty of Biology Publishing
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865
Email: biologi@unsoed.ac.id

This website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version 2.4.8.0.

All article content metadata are registered to:
Crossref
An official nonprofit Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list »