PRODUKSI MISELIUM Grifola frondosa (Dickson: Fries) Gray ISOLAT CIANJUR DAN BOBOT EKSTRAKNYA PADA MEDIUM MYPB DENGAN PENAMBAHAN BIJI BUNGA MATAHARI Helianthus annuus L.

Rizki Maulida, Nuniek Ina Ratnaningtyas, Slamet Priyanto


Abstract


Grifola frondosa or maitake, not only can be used as a food ingredient but also as medicine. Other than nutrients, fruit body and mycelium of G. frondosa also contains bioactive compounds, such as terpenoids, alkaloids, flavonoids, and beta glucans extracellular polysaccharides (β-1,3 glucans and β-1,6 glucans). Extracellular polysaccharide harvested more often in mycelium form which is cultivated in liquid medium. Liquid medium which is commonly used for the growth of mycelium is MYPB (Malt Yeast Peptone Broth). Mycelium production on MYPB as a medium can be increased by adding additional ingredients, one of which is sunflower (Helianthus annuus L.). The treatment was the addition of sunflower seeds on medium MYPB. The main parameters were mycelium’s dry weight and the weight of raw extract of G. frondosa. Supporting parameters were final pH medium, extracellular polysaccharides (qualitatively), terpenoid, alkaloid, and flavonoid compounds in the raw extract of G. frondosa. The addition of 250 g/l sunflower seeds in the medium MYPB was the optimum treatment that can produce 1,379 g/100ml of mycelium and 0,299 g/100ml G. frondosa extracts.


Keywords


extracellular polysaccharides; Grifola frondosa; sunflower seeds

References


Bae JT, Sim GS, Lee DH, Lee BC, Pyo HB, Choe TB, Yun JW. 2005. Production of exopolysaccharide from mycelia culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase-1 expression in uv-irradiated human dermal fibroblast. FEMS Microbiology Letters. 251: 347-354.

Besler M, Hefle SL, Jensen-Jarolim E. 2001. Allergen data collection : sunsflower seed (Helianthus annuus). Internet Symposium on Food Allergens. 3(2): 103-114.

Curvetto N, González-Matute R, Figlas D, Delmastro S. 2002. Sunflower seed-based medium for growth of Ganoderma spp. Mushroom Biology and Mushroom Products. ISBN 968-878-105-3 : 205-211.

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determinationof sugars and related substances. Analytical Chemistry. 28(3): 3550-3560.

Fardiaz S. 1993. Fisiologi Fermentasi. PAU Pangan dan Gizi Bogor: IPB.

Griffin DH. 1994. Fungal Physiology 2nd Edition. New York : Willey-Liss, Inc.

Hadioetomo RS. 1993. Mikrobiologi Dasar dalam Praktek : Teknik dan Prosedur Dasar Laboratorium. Jakarta: Gramedia.

Harborne JB. 1984. Metode Fitokimia. Penuntun Cara Modern Menganalisi Tumbuhan. Bandung: ITB.

Herlina M. 2005. Pengaruh penambahan biji bunga matahari pada media GMC dengan konsentrasi dan waktu inkubasi berbeda terhadap pertumbuhan muselium dan produksi polisakarida Ganoderma lucidum [skripsi]. Universitas Jenderal Soedirman-Purwokerto.

Lee BC, Bae JT, Pyo HB, Choe TB, Kim SW, Hwang HJ, Yun JW. 2003. Biological activities of the polysaccharides produced from submerged culture of the edible basidiomycetes Grifola frondosa. Enzyme and Microbial Technology. 32: 6574-6581.

Lin ES. 2010. Production of exopolysaccharides by submerged mycelial culture of Grifola frondosa TFRI1073 and their antioxidant and antiproliferative activities. World Journal Microbiology Biotechnology. 27(3): 555-561.

Postemsky P, González-Matute R, Figlas D, Curvetto N. 2006. Optimizing Grifola sordulenta and Grifola gargal in agar and liquid nutrient media. Micologia Aplicada International.18(1):7-12.


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