KARAKTERISASI BEBERAPA STRAIN GURAMI Osphronemus gouramy Lac. MENGGUNAKAN MARKA RAPD
Giant gouramy, Osphronemus gouramy Lac. is a popular fish species in Indonesia, especially in Java and Sumatera as this freshwater fish species has a high economic value of stable price. Fish farmers in Bogor divide giant gouramy into six strains based on egg productivity, growth rate, and maximum weight of the adult. They are soang, jepang, blue saphire, paris, bastar, and porselin. These various strains lead to the need of study on the genetic relationship among them, which can be performed by the use of RAPD (Random Amplified Polymorphic DNA) marker. This study aims to determine primers producing consistent and polymorphic RAPD markers, determine specific RAPD markers, and know the genetic relationship among several giant gouramy strains. The strains used in this study are soang, jepang, and blue saphire. Survey method was applied employing purposive random sampling technique. Total genomic DNA was isolated using Genjettm genomic DNA purification kit (Fermentas), which was then used as template to amplify RAPD markers with primers OPA-07, OPA-09, OPA-11, OPA-20, OPAH-01, OPAH-08, OPAH-09, and OPAC-14. The variables examined were patterns and numbers of specific DNA fragments as the PCR amplification products. Selected primers were determined descriptively on the basis of specific DNA bands appearing on the agarose gel. Genetic diversity was predetermined by changing qualitative band pattern into quantitative binnary data. Genetic relationship was analyzed using cladistic method with PAUP software. The results showed that only five of the eight primers produce consistent and polymorphic RAPD markers, i.e. OPA-11, OPA-20, OPAH-1, OPAH-8, and OPAH-9. Specific RAPD markers which can be used to distinguish several gouramy strains are those amplified with OPA-20 of 786 bp, OPA-20 of 1,176 bp, OPAH-8 of 1,000 bp and OPAC-14 of 1,607 bp. Nervertheless, it was found that RAPD markers cannot be used to clearly determine genetic relationship among gouramy strains.
Allard RW. 1960. Principle of Plant Breeding. New York: Willey and Son. Inc.
Asih S, Nugroho E, Mulyasari. 2006. Penentuan keragaman genetik ikan batak (Tor sorro) dari Sumatera Utara dengan metode Analisis Random Amplifified Polymorphism DNA (RAPD). Balai Riset Perikanan Budidaya Air Tawar; Kumpulan Hasil Riset Tahun 2006.
Dharmayanti NLPI. 2011. Filogenetika molekuler: metode taksonomi organisme berdasarkan sejarah evolusi. Wartazoa. 21(1):1-10
Dodgson JB, Cheng HH, Okimoto R. 1997. DNA marker technology: a revolution in animal genetics. Poultry Science. 76:1108-1114. http://dx.doi.org/10.1093/ps/76.8.1108
Fitch WM. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Systematic Zoology. 20:406-416. http://dx.doi.org/10.2307/2412116
Hidayat T, Kusumawaty D, Kusdianti DD, Yati AA, Muchtar, Mariana D. 2008. Analisis filogenetik molekuler pada Phyllanthus niruri L. (Euphorbiaceae) menggunakan urutan basa DNA daerah Internal Transcribed Spacer (ITS). Jurnal Matematika dan Sains, 13(1):16-21.
Iwan D. 2006. Budidaya Gurami. Jakarta: Penebar Swadaya.
Jain A, Bhatia S, Banga SS, Prakash S. 1994. Potential use of random amplified polymorphic DNA (RAPD), technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theoritical and Applied Genetics. 88:116-122. http://dx.doi.org/10.1007/BF00222403
Kottelat M, Whitten AJ, Kartikasari SN, Wirjoatmodjo S. 1993. Freshwater Fishes of Western Indonesia and Sulawesi. Republic of Indonesia: Periplus ed Ltd.
Kludge AG, Farris JS. 1969. Quantitative phyletics and the evolution of anurans. Systematics Zoology. 18:1-32. http://dx.doi.org/10.2307/2412407
Lestari E. 2011. Variasi genetik ikan sepat (Trichogaster trichopterus) di bagian hilir sungai karang rowo undaan kudus berdasarkan polimorfisme marka RAPD [Skripsi]. Universitas Jenderal Soedirman-Purwokerto.
Muneer PMA, Gopalakrishnan A, Musammilu KK, Mohindra V, Lal KK, Basheer VS, Lakra WS. 2009. Genetic variation and population structure of endemic yellow catfish, Horabagrus brachysoma (Bagridae) among three population of western ghat region using RAPD and microsatellite markers. Molecular Biology and Reproduction. 36:1779-1791. http://dx.doi.org/10.1007/s11033-008-9381-6
O'Brien SJ. 1991. Mammalian genome mapping: lessons and prospects. Current Opinion in Genetic Development. 1:105-111. http://dx.doi.org/10.1016/0959-437X(91)80050-V
Sitanggang M, Sarwono B. 2007. Budidaya Gurami Edisi Revisi. Jakarta: Penebar Swadaya.
Welsh J, McClelland M. 1990. Fingerprinting genome using PCR with arbitary primers. Nucleic Acid Research. 18:7213-7218. http://dx.doi.org/10.1093/nar/18.24.7213
William DS, Colome JS, Cano RJ. 1996. Schaum's Outline of Theory and Problems of Molecular and Cell Biology. USA: The McGraw-Hill.
Williams JGK, Kubelic AR, Livak JK, Ravalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitary primer are useful as genetic marker. Nucleic Acid Research. 18:6531-6539. http://dx.doi.org/10.1093/nar/18.22.6531
Article ReadsTotal: 1275 Abstract: 641 PDF: 634
Metrics powered by PLOS ALM
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
All article content metadata are registered to:
An official nonprofit Registration Agency of the International Digital Object Identifier (DOI) Foundation.
Articles in this journal are indexed by: