Email this article RESPON AKTIVITAS AMILASE DAN PROTEASE IKAN GURAMI Osphronemus gouramy Lac. TERHADAP PERBEDAAN TEMPERATUR AIR
, Untung Susilo (2) , Slamet Priyanto (3)
(1) Fakultas Biologi, Universitas Jenderal Soedirman
(2) Fakultas Biologi, Universitas Jenderal Soedirman
(3) Fakultas Biologi, Universitas Jenderal Soedirman
Abstract
Research to determine the activity of amylase and protease digestion gourami on various temperature has been carried out experimentally using a completely randomized design (CRD) with 3 treatments and 4 replications. The treatments were included gourami nurturing on water temperature 24oC (P1), gourami nurturing on water temperature 28oC (P2), and gourami nurturing on water temperature 32oC (P3). The results the amylase activity is showed no significant difference between treatments (P>0,05) where as protease activity showed there are significant differences between treatments (P0≤0,05). In conclusion, the difference in protease activity of gourami occured in water temperature 24oC (P1) and 28oC (P2) and the protease activity was highest at 28oC water temperature, but the amylase activity did not difference.
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Affandi R, Sjafei DS, Raharjo MF, Sulistiono. 2005. Fisiologi ikan, pencernaan, dan penyerapan makanan. Bogor: Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Budiyati R, Palestina S, Nicho A, Sandra M. 2009. Pengukuran daya cerna pati secara in vitro, pengukuran kadar serat pangan metode enzimatik-gravimetrik, dan pengukuran kadar pati resisten. Bogor: Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Bundit T, Bonnie JS, Thomas C, Stephen AS. 2000. Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia, Oreochromis niloticus L. Aquaculture 182: 317–327.
Carman O, Maftucha L, Arfah H. 2006. Pemijahan secara buatan pada ikan gurame Osphronemus gouramy Lac. dengan penyuntikan ovaprim. Jurnal akuakultur Indonesia 5(2): 103-112.
Fernando L, Garcia C, Cristiane ACM, Angeles NT, Evoy Z. 2007. Digestive proteinases of Brycon orbignyanus (Characidae Teleostei):characteristics and effects of protein quality. Comparative Biochem. and Physiol. Part B 132: 343–352
Furne M, Gallego GM, Hidalgo MC, Morales AE, Domezain A, Domezaine J, Sanz A. 2005. Digestive enzyme activities in adriatic sturgeon (Acipenser naccarii) and raibow trout (Oncorhynchus mykiss). Comparative Biochem. and Physiol. Aquculture 250: 391-398.
Grizlle JM, Rogers WA. 2003. Anatomy and histology of the freshwater. Departement of Fisheries and Applied Aquaculture, Auburn University. p. 89.
Handayani S, Zairin M, Mokoginta I, Bintang M, Sudrajat AO. 2005. Perubahan enzim-Enzim pencernaan pada ikan gurami (Osphronemus gouramy) sebagai respon terhadap pakan yang mengandung kadar protein dan karbohidrat serta suhu yang berbeda. Aquakultura Indonesiana 9(1): 25-29.
Hermanto. 2000. Optimalisasi suhu media pada pemeliharaan benih ikan gurami (Osphronemus gouramy Lac.) [tesis]. Program Pascasarjana. Bogor: Institut Pertanian Bogor. p. 63.
Hidalgo MC, Urea E, Sanz A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. proteolytic and amylase activities. Aquaculture 170(3): 267-283.
Kelabora DM. 2010. Pengaruh suhu terhadap kelangsungan hidup dan pertumbuhan larva ikan Mas (Cyprinus carpio). Berkala Perikanan Terubuk 38(1): 13.
Kostati R. 2006. Konsumsi pakan maksimal dan pertumbuhan ikan gurami pada kondisi suhu yang berbeda [skripsi]. Bogor: Program Studi Budidaya Perairan, Fakultas Perikanan, Institut Pertanian Bogor. p. 65.
Kurnia DRD. 2010. Studi aktivitas enzim pencernaan dari Osphronemus gouramy sebagai biokatalis pada proses gliserolisis untuk menghasilkan monoasilgliserol [tesis]. Program Pascasarjana. Semarang: Universitas Diponegoro. p. 72.
Moyle PB, Joseph CJ. 2001. Fishes an introduction to ichtiology. Fourth Edition. London: Prentice-hill International Limited.
Nasir M. 2002. Pengaruh kadar selulosa yang berbeda dalam pakan terhadap panjang usus dan aktivitas enzim pencernaan benih ikan gurami [tesis]. Bogor: Program Pasca Sarjana, Institut Pertanian Bogor.
Natalia Y, Hasyim R, Ali A, Chong A. 2004. Characterization of digestive enzymes in a carnivorous ornamental fish, the Asia Bony Tongoe, Sceleropages formosus (Osteoglossidae). Aquaculture 233(3): 305-320.
Nathanailides C. 2006. Are changes in enzyme activities of fish muscle during cold acclimation significant. National Centre for Marine Research Aquaculture Unit. Departement of Veterinary Basic Science, University of London. London: NWI OTU U.K.
Papoutsoglou ES, Lyndon AR. 2002. Effect of incubation on enzyme digestion in important teleost for aquaculture. Aquaculture 27: 1243-1257.
Papoutsoglou ES, Lyndon AR. 2005. Effect of incubation temperature on carbohydrate digestion in important teleost for aquaculture. Aquaculture Research 36: 1252-1264.
Rahayu R. 2002. Efisiensi dan konversi pakan pada ikan gurami yang dipelihara dengan temperatur air yang berbeda [skripsi]. Purwokerto: Fakultas Biologi Universitas Jenderal Soedirman.
Rome LC, Funke RP, Alexander RM. 2004. The influence of temperature on muscle velocity and sustained performance in swimming carp. J. Exp. Biol. 154: 163-178.
Schindler DW. 2001. The cumulative effects of climate warming and other human stresses on Canadian freshwaters in the new millennium. Department of Biological Sciences. J. of Fish Aquatic 58: 18–29.
Sebayang F. 2005. Isolasi dan pengujian aktivitas enzim a- amilase dari Aspergillus niger dengan menggunakan media campuran onggok dan dedak. J. Komunikasi Penelitian. 17 (5).
Setyowati DN, Hardiningsih I, Priyono SB. 2007. Sintasan dan pertumbuhan benih pasca larva beberapa subspesies gurami (Osphronemus gouramy). J. Perikanan 9(1): 149-153.
Steel RGD, Torrie JH. 1993. Prinsip dan prosedur statistika. Jakarta: Gramedia Pustaka Utama.
Syamdidi, Ikasari D, Wibowo S. 2006. Studi sifat fisiologi ikan gurami (Osphronemus gourami) pada suhu rendah untuk pengembangan teknologi transportasi ikan hidup. J. Pascapanen dan Bioteknologi Perikanan 1(1)
Tranggono, Sutardi. 2005. Sifat fisiologi dan biokimiawi ikan gurami. Yogyakarta: Gadjah Mada University Press.
Wijayanti. 2004. Studi aktivitas protease pada benih ikan gurami [skripsi]. Bogor: Fakultas Perikanan Institut Pertanian Bogor.
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