TITER EKDISON LOBSTER HIJAU PASIR (Panulirus homarus L.) PADA FASE PREMOLTING AKHIR
Spiny Lobster, Panulirus homarus, farming is very potential to be developed in Lombok Island, but physiological aspects such as nutrient needs and growth hormone, especially ecdysone titer, were little known. This research aimed to determine the ecdysone titer of Panulirus homarus during the last pre-molting phase. This study was conducted as a quasi-experiment with one group post-test design. The lobsters used in this experiment have the length of carapacs and the body weight of 1.5–2.0 cm and 4.33–5.77 g respectively. The lobster fed for 42 days with gel pellets three times a day before ecdysone titer measurement. The ecdysone hormone was then isolated from the second segment of the abdomen on the basal of the fifth pereiopod. Ecdyson titer was measured by using High-Performance Liquid Chromatography (HPLC). The result showed that the titer of ecdysone was 5.27 µg/ml hemolymph. The titer of ecdysone was found higher compared to previous research on Homarus americanus; this result suggested that the spiny lobster Panulirus homarus needs a higher amount of ecdysone hormone to initiate the molting process.
Ahmad I, Rahayu R, Permana AD, Astari S. 2007. Alteration of ecdisteroid titre by thyroxine and juvenile hormone analogue (methoprene) in Bombyx mori (Lepidoptera : Bombycidae). Biota. 12(2): 116–121.
Chang ES, Sharon AC, Eva PM. 2001. Hormones in the lives of crustaceans: an overview. AMER. Zool. 41:1090–1097. https://doi.org/10.1093/icb/41.5.1090
Chapman RF. 1998. The insect: structure and function 4th edition. UK: Cambridge university press. https://doi.org/10.1017/CBO9780511818202
Faturrahman. 2012. Penggunaan puding gracilaria sebagai karir feed additive untuk abalone. Simposium nasional bioteknologi akuakultur IV di Bogor.
Houser RH, Akiyama DM. 1997. Feed formulations principles. In D'Abramo LR, Conklin DE, Akiyama DM. Crustacean nutrition, advances in world aquaculture. Vol.6. USA : International Working Group on Crustacean Nutrition, World Aquaculture Society. 587 p.
Ihsan M, Trijoko, Widjayanti N. 2013. Pengaruh penambahan silase artemia dalam pelet gel terhadap pertumbuhan, persentase molting dan titer ekdison lobster hijau pasir (Panulirus homarus) pada fase juvenil (Tesis). Fakultas biologi UGM. Yogyakarta.
Imayavaramban L, Dhayaparan, Halagowder D. 2007. Molecular mechanism of molt-inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y-organ of mud crab: Scylla serrata. FEBS Letters. 581:5167–5172. https://doi.org/10.1016/j.febslet.2007.09.029
Pankotai T, Popescu C, Martin D, Grau B, Zsindley B, Bodai L, Tora L, Ferrus A, Boros I. 2010. Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila. Molecular and cellular biology. 30:4254–4266 https://doi.org/10.1128/MCB.00142-10
Philips BF. 2006. Lobsters: Biology, management, aquaculture and fisheries. USA: Blackwell Publishing Ltd. 506 p https://doi.org/10.1002/9780470995969
Prahasto D, Probandari A. 2017. Rancangan penelitian eksperimen murni dan kuasi-eksperimental. Yogyakarta: Universitas Gadjah Mada (UGM). [Internet] Available from http://gamel.fk. ugm.ac.id/pluginfile.php/72599/mod_folder/content/0/materi/Materi%2021a%20Penelitian%20Kuasi%20Eksperimental%20dan%20Eksperimental.pdf?forcedownload=1
Priyambodo B. 2011. Seed census & puerulus assessment techniques, seasonality, sustainability in Indonesia. Annual meeting lobster research di Lombok.
Smith DM, Irvin SJ, David M. 2009. Optimising the physical form and dimension of feed pellets for tropical spiny lobsters. Dalam K.C. Williams. Spiny lobster aquaculture in the Asia-Pacific region. ACIAR Proceedings 132: Canberra; P. 157–162.
Spaziani E, Thomas CJ, Wenan LW, Jeffrey AB, Shanon MC, Corey CC, Matt JD, Christopher MS, Danice KS, Rex M. 2001. Further studies on signaling pathways for ecdysteroidogenesis in crustacean Y-organs. AMER. ZOOL. 41:418–429. https://doi.org/10.1093/icb/41.3.418
Supriyono E, Prihardianto RW, Nirmala K. 2017. The stress and growth responses of spiny lobster Panulirus homarus reared in recirculation system equipped by pvc shelter. AACL Bioflux. 10(2): 147-155.
Waterman TH. 1961. Sense organ, integration, and behavior vol. 1. New York & London : Academic Press. 350 p
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