PENGARUH PEPTON TERHADAP PERTUMBUHAN EMBRIO ANGGREK Vanda tricolor Lindley var. Suasiv ASAL MERAPI SECARA IN VITRO

Rizka Riliant Puspasari, Ikhsanudin Nur Rosyidi, Eka Fitriana Candra Ningrum, Endang Semiarti


Abstract


Abstract

Orchidaceae is one of the important biodiversity that included in the national commodity. The growth of orchids in nature are very low due to the microscopic size of the seed and does not have endosperm that needs to be done in vitro culture with the addition of the inducer, one of which is peptone. Orchid embryos used is Vanda tricolor Lindley var. Suavis Endemic Merapi. This study aims to determine the effect of peptones on the growth of embryos orchid V. tricolor 8 weeks and optimal growth for the orchid embryo induction V. tricolor with peptone. This research was conducted in the laboratory of Biotechnology Faculty of Biology in September-October 2016. The research method that is making peptone medium with varying concentrations (0-3 g/L), a subculture protocorm 8 weeks old, and a morphological analysis protocorm orchids. The results showed peptone positive effect on the growth of embryos orchid V. tricolor in peptone concentration of 1 g/L (NP1) with the growth rate reaching phase 6 in the fourth week of 6.85 ± 0.001%. This value is higher than the growth rate protocorm when it reaches phase 6 on the fourth week of medium NP0 of 5.22 ± 0.003%, the medium NP2 of 1.15 ± 0.002%, and the medium NP3 of 1.02 ± 0.001 %. It shows that the optimal concentration of peptone can support the growth of the orchid embryo V. tricolor, but the concentration of peptone excess can inhibit the growth of orchids protocorm V. tricolor.

Key Words: In vitro, Orchid, peptone, protocorm, V. tricolor

Keywords


Bioteknologi; In vitro; Orchid; peptone; protocorm; Vanda tricolor

Article Reads

Total: 46 Abstract: 46

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

This website is maintained by:
The Faculty of Biology Publishing 
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865
Email: biologi@unsoed.ac.id

This website uses: 
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version 2.4.8.0.

All article content metadata are registered to:
Crossref
An official nonprofit Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list »