BIODEGRADATION OF DIESEL OIL BY YEAST ISOLATED FROM MANGROVE’S RHIZOSPHERE

Ryan Firman Syah (1) , Agus Irianto (2) , Nuniek Ina Ratnaningtyas (3)


(1) Faculty of Health, Universitas Mohammad Husni Thamrin
(2) Faculty of Biology, Universitas Jenderal Soedirman
(3) Faculty of Biology, Universitas Jenderal Soedirman


Abstract


Diesel oil-degrading yeast strains isolated from mangrove rhizosphere at Tritih Kulon, Cilacap had been screened with SMSS medium. Four culturable yeast were isolated. Qualitative test was conducted by culturing and incubating the yeasts for one month in a medium added with 1mL diesel oil. By measuring the reduction of diesel oil, two best yeasts were selected. The quantitative test, GC-MS analysis, was conducted to determine the detailed degradation process of diesel oil. Candida lusitaniae and Cryptococcus laurentii performed the degradative ability. Three highest percent area of hydrocarbon compounds were compared for assessment. The results showed that C. lusitanie had better degradative capability than C. laurentii, in which hexadecane and methyl hexadecanoate decreased by 90–95%, and 9-octadecenoic acid, methyl ester declined by 30–40%. The increasing pH medium during incubation suggested that fermentation process occurred.


Keywords


yeast; diesel oil-degrading; hydrocarbon compound

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References


Al-Tahhan RA, Sandrin TR, Badour AA, Maier RM. 2000. Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa: effect on cell surface properties and interaction with hydrophobic substrates. Applied and Environment Microbiology, 66(8):3262–3268 https://doi.org/10.1128/AEM.66.8.3262-3268.2000

Chandran P, Das N. 2010. Biosurfactant production and diesel oil degradation by yeast species Trichosporon asahii isolated from petroleum hydrocarbon contaminated soil. International Journal of Engineering Science and Technology, 2(12): 6942–6953

Caraka Online [Internet]. c2000-2003. Menyelamatkan Hutan Mangrove Cilacap [cited 2012 Jun 09]. Available from: http://cilacap-online.tripod.com/hutanbakau.htm

Haddock JD. 2010. Aerobic degradation of aromatic hydrocarbons: enzyme structures and catalytic mechanisms. In: Timmis KN (Ed.). Handbook of Hydrocarbon and Lipid Microbiology. p.1057–1069. Springer, Berlin https://doi.org/10.1007/978-3-540-77587-4_74

Irianto A, Oedjijono, Riyanto A, Komar MS. 2003. Bioaugmentasi benzena tanah tercemar hidrokarbon yang dibiodegradasi secara in vitro dengan menggunakan Bacillus sp. strain U41 dan U44. Biota, 8(3):101–106

Leahy JG, Colwell RR. 1990. Microbial degradation of hydrocarbons in environment. Microbiol Rev, 54(3):305–315

Miranda RdC, de Souza CS, Gomes EdB, Lovaglio RB, Lopes CE, Sousa MFVdQ. 2007. Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco-Brazil. Braz Arc Biol Technol, 50(1):147–152 https://doi.org/10.1590/S1516-89132007000100018

Nurhariyati, 2004. Biodegradasi heksadekana oleh Candida sp. hasil isolasi dari Pelabuhan Tanjung Perak Surabaya [laporan penelitian]. Airlangga University Library: Surabaya

Pribadi R, Hartati R, Suryono CA. 2009. Komposisi jenis dan distribusi gastropoda di kawasan hutan mangrove Segara Anakan Cilacap. Ilmu Kelautan, 14(2):102–111

Ramadan KMA, Azeiz AZA, Hassanien SE, Eissa HS. 2012. Biodegradation of used lubricating and diesel oils by a new yeast strain Candida viswanathii KA-2011. African Journal of Biotechnology, 11(77):14166–14174 https://doi.org/10.5897/AJB12.1339

Sabarni N. 1995. Kemampuan Pseudomonas fluorescens FNCC 0070 dalam biodegradasi toluena (C7H8) dengan penambahan urea sebagai sumber nitrogen [undergraduate thesis]. Fakultas Biologi Universitas Jenderal Soedirman: Purwokerto

Suradi, Sukimin S, Boer M, Affandi R, Monintja DR. 2005. Aspek reproduksi dan daerah pemijahan udang jari (Metapenaeus elegans de Man, 1907) di Laguna Segara Anakan, Cilacap, Jawa Tengah. IJMS, 1 (1):41–49

Setyawan AD, Winarno K. 2006. Pemanfaatan langsung ekosistem mangrove di Jawa Tengah dan penggunaan lahan di sekitarnya; kerusakan dan upaya restorasinya. Biodiversitas, 7(3):282–291 https://doi.org/10.13057/biodiv/d070318

Soedradjad R. 2000. Kadar pencemaran minyak, fenol di estuari Sungai Donan, Cilacap [laporan penelitian]. Universitas Jember. Jember: Jember. p.44–58

Soedradjad R. 2003. Fungsi model hidrodinamika dalam pengelolaan ekosistem mangrove (studi kasus pencemaran minyak di estuari Sungai Donan Cilacap). Berkala Penelitian Hayati, 8(2):81–84

Sood N, Patle S, Lal B. 2010. Bioremediation of acidic oil sludge-contaminated soil by the novel yeast strain Candida digboiensis TERI ASN6. Environ Sci Pollut Res Int, 17(3):603–610 https://doi.org/10.1007/s11356-009-0239-9

Vater J, Kablitz B, Wilde C, Franke P, Mehta N, Cameotra SS. 2002. Matrix assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Appl Environ Microbio, 68(12):6210–6219 https://doi.org/10.1128/AEM.68.12.6210-6219.2002


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