Pemakaian Sel Hela dalam Uji Sitotoksisitas Fraksi Ethanol Biji Mimba (Azadirachta indica)

Abstrak

Traditionally, the neem (Azadirachta indica) seeds have been used as bioinsecticide. It was also used and known for long time as a fungicide and anti bacterial agents. Pre-toxicity experiment using brine shrimp lethality test (BSLT) method indicated that the ethanol fraction of the neem seeds was toxic to the shrimp larvae with LC50 38.79 μg/ml. This experiment was done to further investigate the possible role of ethanol fraction of neem (A. indica) seeds as an anti cancer agent using HeLa cell lines. Experiment was initiated by extracting the seeds using n-hexane by maceration. The macerate was then macerated further using 70% ethanol as solvent. The ethanol fraction was used in cytotoxicity assay using (cervix cancer) HeLa cell lines. Cytotoxicity assay was done by using direct counting method. The amount of living cells were observed and counted, death percentage was determined and probit analysis used to determine the LC₅₀ value. LC₅₀ value for ethanol fraction from the seeds of neem (A. indica) was 10.95 μg/ml for 24 hours; 7.33 μg/ml for 48 hours and 4.37 μg/ml for 72 hours based on cytotoxicity analysis using HeLa cells. This experiment concluded that the neem (A. indica) seeds have cytotoxicity toward HeLa cell lines which confirmed the previous toxicity experiment using brine shrimp lethality test (BSLT) method. Overall, this experiment indicated that neem (A. indica) seeds have anticancer activity.