Pengaruh Pemberian Sitokinin Terhadap Pertumbuhan Palea dan Lemma Padi Melaui Kultur In Vitro
A research has been carried out with the aims to: 1. study the influence of both the kind and concentration of cytokinins on palea and lemma growth in in vitro culture; 2. study the influence of palea and lemma age towards external application of cytokinin in in vitro culture; and3. determine the best palea-lemma age, kind and concentration of cytokinin which resulted the best growth of palea and lemma in in vitro culture. The used experimental method was Completely Randomised Design (CRD) with factorial treatment pattern. The applied treatment consisted of three factors i.e. palea-lemma age (U): (55, 60, and 65 days after planting); kind of cytokinin (S): (BAP and Kinetin); and Cytokinin Concentration (K): (0, 5, 10, and 15 M) with 3 replications. The observed variables were the palea and lemma growth with the parameters were palea-lemma length and width. The results showed that the kind of cytokinin had influenced the growth of palea-lemma in in vitro culture, and kinetin has better influence on the palea-lemma growth. The age of the palea-lemma determined the responsiveness of the palea-lemma towards external application of cytokinin. Older palea-lemma showed less responsive than younger ones towards external application of plant growth regulators. Moreover, the treatment combination (U1S2K2) (10 µM Kinetin applied to 55-day-old rice palea-lemma) had the best effect on increasing the size of palea-lemma of IR 64 rice.
Ebenezer, G.A.I., Amirthalingham, M., Ponsamuel, J., and Dayanandan, P., 1990. Role of palea and lemma in the development of rice caryopsis. Journal of Indian Botany Society 69: 245 – 250
Ebenezer, G.A.I., Krishnan, S., and Dayanandan, P., 2001. Structure of rice caryopsis in relation to strategies for enhancing. In Peng, S. and Hardy, B. (editors). Rice Research For Food, Security and Poverty Alleviation. Proceeding of the International Rice Research Conference, 31 March – 3 April 2000. Los Banos Philippines. Los banos (Philippines). International Rice Research Institute. 692 p.
Jacobs, T., 1992. Reviews: Control of the Cell Cycle. Developmental Biology 153: 1 – 15.
Khush, G.S., 1997. Origin, dispersal, cultivation of rice. Plant Molecular Biology 35: 25 – 34.
Krishnamoorthy, H.N., 1981. Plant growth substances including applications in agriculture. Tata McGraw-Hill Publishing Company Limited, New Delhi.
Krishnan, S., Azhakanandam, K., Ebenezer, G.A.I., Samson, N.P., and Dayanandan, P., 1999. Brassinosteroids and benzulaminopurine increase yield in IR50 indica rice. Current Science 76(2): 145 – 147.
Liu, C.N., Li, X.Q., and Gelvin, S.B., 1992. Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens. Plant Molecular Biology 20: 1071-1087.
Ordas, R.J., Fernandez, B., and Rodriguez, R., 1992. Benzyl Adenin Controled Protein Synthesis and Growth in Apple Cell Suspensions. Physiologia Plantarum 84 (2): 229-235.
Tran, D.V., 2001. Closing the rice yield gap for food security. In. Peng, S., and Hardy, B., (Edtors). Rice research for food security and poverty alleviation. Proceeding of the International Rice Research Conference, 31 March – 3 April 2000, Los Banos, Philippines. Los Banos (Philippines): International Rice Research Institute. 692 p.
Wareing, P.F., and Phillips, I.D.J., 1981. Growth and differentiation in plant (3rd edition). Pergamon Press, Oxford.
Wattimena, G.A., 1987. Diktat Zat Pengatur Tumbuh Tanaman. Laboratorium Kultur Jaringan Tanaman PAU Bioteknologi IPB, Bogor.
Article ReadsTotal: 2637 Abstrak: 421 PDF: 2216
Metrics powered by PLOS ALM
- Saat ini tidak ada refbacks.
Jumlah Kunjungan Harian
Satu bulan terakhirTampilkan laporan lengkap Google Analyticsdi sini
Metadata artikel terdaftar di:
Agen resmi internasional pendaftaran Digital Object Identifier (DOI)
Artikel jurnal ini terindeks: