Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)

Herdis Herdis; Maman Surachman; Muhammad Rizal; Arief Boediono; Yulnawati Yulnawati

doi:10.20884/1.mib.2006.23.1.142

Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries)

Herdis Herdis⁽¹⁾

, Maman Surachman⁽²⁾ , Muhammad Rizal⁽³⁾ , Arief Boediono⁽⁴⁾ , Yulnawati Yulnawati⁽⁵⁾

⁽¹⁾
⁽²⁾
⁽³⁾
⁽⁴⁾
⁽⁵⁾

DOI: 10.20884/1.mib.2006.23.1.142

Abstrak

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations. Cement was collected from three mature rams using an artificial vagina once a week. After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively. The extended-cement was stored at 5^oC. Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days. The results showed that addition of trehalose did not significantly improved the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5^oC for 4 days. In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.

Kata Kunci

Trehalose, Tris extender, chilled-cement, Garut ram

Teks Lengkap:

PDF

Referensi

Aboagla, E.M.E. and T. Terada. 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod. In Press.

Aisen, E.G., H.L. Alvarez, A. Venturino, and J.J. Garde. 2000. Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology 53:1053-1061.

Aisen, E.G., V.H. Medina, and A. Venturino. 2002. Cryopreservation and post-thawed fertility of ram frozen semen in different trehalose concentrations. Theriogenology 57:1801-1808.

Farhan. 2003. Kajian Nira sebagai Pengencer Alternatif Semen Domba Garut. Skripsi. Fakultas Peternakan, Institut Pertanian Bogor, Bogor.

Feradis. 1999. Penggunaan Antioksidan dalam Pengencer Semen Beku dan Metode Sinkronisasi Estrus pada Program Inseminasi Buatan Domba St. Croix. Disertasi. Program Pascasarjana, Institut Pertanian Bogor, Bogor.

Herdis. 2005. Optimalisasi Inseminasi Buatan Melalui Aplikasi Teknologi Laserpunktur pada Domba Garut (Ovis aries). Disertasi. Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor.

Inounu, I., N. Hidajati, S.N. Jarmani, D. Priyanto, Hastono, B. Setiadi, dan Subandriyo. 2001. Pengaruh interaksi genetik dan lingkungan terhadap produksi domba persilangan dan domba lokal pada beberapa lokasi pengamatan: evaluasi kualitas semen domba hasil persilangan. Prosiding Seminar Hasil Penelitian Bagian “Proyek Rekayasa Teknologi Peternakan/ARMP II”. Puslitbang Peternakan. Hlm 64-73.

Kristanto, T. 2004. Peranan Gliserol dan Fetal Bovine Serum dalam Pengencer Tris Kuning Telur terhadap Kualitas Semen Cair Domba Garut. Skripsi. Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.

Revell, S.G. and R.A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36:77-86.

Rizal, M., M.R. Toelihere, T.L. Yusuf, B. Purwantara, dan P. Situmorang. 2003. Kriopreservasi semen domba garut dalam pengencer Tris dengan konsentrasi laktosa yang berbeda. Media Kedokteran Hewan 19:79-83.

Steel, R.G.D. dan J.H. Torrie. 1993. Prinsip dan Prosedur Statistika. Gramedia Pustaka Utama, Jakarta.

Storey, B.T., E.E. Noiles, and K.A. Thompson. 1998. Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology 37:46-58.

Subowo. 1995. Biologi Sel. Angkasa, Bandung.

Toelihere, M.R. 1993. Inseminasi Buatan pada Ternak. Angkasa, Bandung.

Woelders, H., A. Matthij, and B. Engel. 1997. Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology 35:93-105.

Yildiz, C., A. Kaya, M. Aksoy, and T. Tekeli. 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing. Theriogenology 54:579-585.

Yulnawati. 2002. Pemanfaatan Sari Buah Melon dan Sari Wortel sebagai Pengencer Alternatif Semen Domba Garut. Skripsi. Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.