Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus)

Agus Nuryanto; Dedy Duryadi Solihin

doi:10.20884/1.mib.2006.23.1.143

Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus)

Agus Nuryanto⁽¹⁾

, Dedy Duryadi Solihin⁽²⁾

⁽¹⁾
⁽²⁾

DOI: 10.20884/1.mib.2006.23.1.143

Abstrak

A study on sequence variation in the mitochondrial DNA of Trochus niloticus has been conducted. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase I gene of 533 bp and ABI 310 aotomatic DNA sequencer was used to sequence the PCR product. This study was aimed to know the genetic variation of T. niloticus and the suitability of partial sequence of cytochrome c oxidase I gene as a molecular marker in the genetic study. The results showed that from two T. niloticus specimens, 12 polymorphic sites were detected yielding 2 haplotypes. This indicated a highly genetic variation in thecytochrome c oxidase I (COI) gene of T niloticus. In addition, cytochrome c oxidase I gene was proved to be a suitable marker for the genetics studies of various species of organisms.

Kata Kunci

sequence variation, Trochus niloticus, cytochrome c oxidase I gene

Teks Lengkap:

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Referensi

Arifin, Z. 1993. Geographical distribution, habitat and fishery of top shell (Trochus niloticus) in Maluku. Perairan Maluku dan Sekitarnya, Ambon 8: 93-101.

Avise, J.C., J. Arnold, R.M. Ball, E. Bermingham, T. Lamb, J.E. Neigel, C.A. Reeb and N.C. Saunders. 1987. Intraspecific phylogeography: the mitochondrial DNA bridges between population genetics and systematics. Ann. Rev. Ecol. Syst. 18: 489-522.

Barber, P.H., S.R. Palumbi, M.V. Erdmann and M.K. Moosa. 2002. Sharp genetic breaks among populations of Haptosquilla pulchella (Stomatopod) indicate limits to larval transport: patterns, causes and consequences. Molecular Ecology 11: 659-674.

Bucklin, A., B.W. Frost, J. Bradford-Grieve, L.D. Allen and N.J. Copley. 2003. Molecular systematic and phylogenetic assessment of 34 calanoid copepod species of the calanidae and clausocalanidae. Marine Biology 142: 3333-3343.

Duran, S., C. Palacin, M.A. Becerro, X. Turon and G. Giribet. 2005. Genetic diversity and population genetic of the commercially harvested sea urchin Paracentrotus lividus (Echinodermata, Echinoidea). Molecular Ecology 13: 3317–3328.

Folmer, O, M. Black, R. Lutz and R. Vrijenhoek. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3 (5): 294-299.

Hebert, P.D.N., S. Ratnasingham and J.R. deWaard. 2003a. Barcoding anmal life: cytochrome c oxidase subunit I divergences among closely related species. Proc. R. Soc. Lond. B (suppl.) 270: s96-s99.

Hebert, P.D.N., A. Cywinska, S.L. Ball and J.R. deWaard. 2003b. Biological identifications through DNA barcodes. Proc. R. Soc. Lond. B 270: 313-321.

Iguchi, K., Y. Tanimura, H. Takeshima and M. Nishida. 1999. Genetic variation and geographic population structure of amphidromus ayu Plecoglosus altivelis as examined by mitochondrial DNA sequencing. Journal Fisheries Sciences 65: 63-67.

Luttikhuizen, P.C., J. Drent and A.J. Baker. 2003. Disjunct distribution of highly divergent mitochondrial lineage clade and population subdivision in marine bivalve with pelagic larval dispersal. Molecular Ecology 12: 2215-2229

Moritz, C., T.E. Dowling and W.M. Brown. 1987. Evolution of animal mitochondrial DNA: relevance for biology and systematics. Ann. Rev. Ecol. Syst. 18: 269-292.

Pet-Soede, L and M. Erdman. 2004. An overview and comparison of destructive fishing practice in Indonesia. http://www.spc.org.nc/coatfish/news/lrf/4/

Erdman.htm, diakses 20 Januari 2004

Randi, E. 2000. Mitochondrial DNA. In J.A. Baker (Ed). Molecular Methods in Ecology. Blackwell Science, Toronto.

Shefer, S., A. Abelson, O. Mokady and E. Geffen. 2004. Red to Mediterranean Sea bioinvasion: natural drift through the Suez Canal, or antrophogenic transport? Molecular Ecology 12: 2333-2343.

Sunnuck, P. 2000. Efficient genetic marker for population biology. TREE 15 (5): 199-203.

Tingey, S.V., J.A. Rafalski and J.G.K. Williams. 1992. Genetic Analysis with RAPD Marker. Joint Plant Breeding Symposia Series, November 1, 1992. Minneapolis, Minnesota.

Walsh, P.S., D.A. Metzger and R. Higushi. 1991. Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10 (4): 506-513.

Zischler, H. 1999. Mitochondrial DNA: Diversity Analysis and Possible Pitfalls. In J.T. Epplen and T. Lubjuhn (Ed). Methods and Tools in Biosciences and Medicine DNA Profiling and DNA Fingerprinting. Birkhauser Verlag Basel, Switzerland.