Penentuan Konduktivitas Listrik dan Frekuensi Karakteristik Sel Ragi dengan Memanfaatkan Proses Dielektroforesis

Much Azam (1) , Wahyu Setia Budi (2) , Hermin Pancasakti (3)


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Abstrak


Dielectrophorestic (DEP), a phenomenon where small particles such as yeast cells are manipulated by non-uniform electric fields. The net dielectrophoretic force is proportional to the polarisability of the cell and cause this cell moving toward higher field. By measuring velocity of the cell on the various frequency of the electric field, it can determine the electrical conductivity and the characteristic frequency of the yeast cells. The results showed that the conductivity of the yeast cell was (10,0 ± 0,4)10-9 Sm-1  and the characteristic frequency was 60 kHz.

Kata Kunci


Dielectrophoresis, cell membrane, electrical conductivity, yeast cell

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Referensi


Azam, M., 2001. Pengaruh gradien medan listrik terhadap kecepatan sel telur ikan pada proses dielektroforesis. Berkala Fisika 4(3).

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Claveland, 1987, Handbook of chemistry and physics. Edisi 67. CRC Press Inc.

Foster,K.R. and Schwan, H.P., 1996. Dielectric properties of tissues. In: Polk, C. and Postow, E. (Eds). Handbook of biologica l: Effect of electromagnetic fields. CRC, Press, Inc., London.

Hughes, M.P, 1999, AC electrokinetics application for Nanotechnology, http://www.foresight.org/Conferences /MNT7/Paper/Hughes/index.html.

Hoeb, M., Radler,J.O., Klein, S., Stutzmann, M., and Brandt, M.S., 2007. Light-induced dielectrophoretic manipulation of DNA. Biophisical Journal 93(3), 1032-1038.

Mahaworasilpa, T.L , Coster, H.G.L., and George, E.P., 1994. Forces on biological cells due to applied alternating (AC) electric fields,dielectrophoresis. Biochimica et Biophysics Acta 1193,118-126.

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