Optimasi Starter dalam Memproduksi Inulinase dan Identifikasi Khamir Inulinolitik BAN - 1 dari Umbi Dahlia (Dahlia variabilis Willd)

Wijanarka Wijanarka (1) , Endang Kusdiyantini (2) , Hermin Hermin (3)


(1) 
(2) 
(3) 


Abstrak


Inulinolitic yeast isolated from dahlia tuber (Dahlia variabilis Willd) of Bandungan-Ambarawa, was found to produce inulinase enzyme (E.C.3.2.1.7).  Under tested fermentation condition, BAN-1 inulinase isolate showed enzymatic activity in dose dependent manner to the starter concentration. A maximum enzymatic activity of 0.582 IU was produced by adding starter concentration 10%. Based on morphological, physiological and biochamichal evaluation it was indentified that  the BAN-1 isolate is Pichia sp.


Kata Kunci


Inulinase; Pichia sp, Dahlia variabilis Willd

Teks Lengkap:

PDF

Referensi


Allains, J.J., Kammou S., Blanc P., Girard C.and Baratti. J.1986. Isolation and Characteristic of Bacterial Strains with Inulinase Activity. Appl. Environ. Microbiol. 52 (50 : 1086-1090).

________; Hoyos-Lopez G., Kammoun S. and Baratti. J. 1987. Isolation and Characterization of Thermophilic Bacterial Strains with Inulinase Activity. Appl. Environ. Microbiol. 53 (5): 942 –945

Baltz. R.H. l996. Strain Improvement. Di dalam Manual of Industrial Microbiology and Biotechnology. Demain, A,I., and Solomon, N.A. (Ed). American Society for Microbiology. Washington.

Barnett, J.A., Oayne R.W. and Yarrow. D. 1990. Yeasts: Characteristics and Identification. 2 nd Edition. Cambridge Univ. Press. Cambridge

Bajpai, P. and Margaritis A. 1987. Characterization of Moleculer Sieve-Bound Inulinase. J. Ferment Technol. 65 (2): 239 – 247.

Bucke.C. l988. Enzymes in Fructose Manufacture. Dalam. Enzymes and food Processeing. Applied. Science. Pub. Ltd.

Byun, S.M. and Nahm B.H. 1987. Production of Fructose from Jerusalem artichoke by Enzymatic Hydrolysis J. Food sci. 43: 1871 – 1873.

Chaplin, M.F. and Kennedy J.F.. 1994. Carbohydrat Analysis; A Practical Approach. 2nd Edition. Oxford University Press. Oxford.

Cruegur, W. and Crueger A.. 1984. Biotechnology: A Texbook o Industral Microbiology. Thomas (Ed) Sinauer Associated, Inc. Sunderland, M.A 01375.

De Lenheer, L. and Hoebregs, H.. 1994. Inulin. Megazyme International Ireland Ltd. Ireland.

Deutscher, M. l990. Guide To Protein Purification. Methods In Enzymology Vol. 182. Academic Press. Inc. Boston. Toronto. Tokyo.

Doty, T. and Vaninen. 1979 The Properties, Manufacture and Uses as an Industrial Raw Material. Dalam : C.G. Birch dan K.J. Parker (ed) Sugar : Science and Technology Appl. Sci Publ. London

Dixon, M and Webb E. 1979. Enzymes. Logman Group Ltd London

Hartiko, H. l994. Biologi Organisme Termofilik. PAU-Bioteknologi UGM. Jogyakarta.

Holz, G and Saunders G. l985. Genetic Modification of Industrial Microorganisms. Didalam Comprehensive Biotechnology. The Principles, Application and Regulation of Biotechnology in Industry, Agryculture and Medicine. Moo-Young, M (Ed) Pergamon Press.

Javadekar, VS, Siva Raman H. and Gokhale D. 1995. Industrial Yeast Strain improvement : Construction of a Highly Flocculent Yeast With a Killer Character by Protoplast Fusion. Jour. Indst. Microbiology 15 : 94-102

Kockova, A and Kratochilova L.. 1990. Yeast and Yeast Like Organism. UCH. Publishers. France. Pp. 6 – 7, 42 – 51.

Kreger-van Rij, N.J.W. 1984. The yeast, A Taxonomic Study. Third revised and Enlarged Ed., Elsevier sci. publ. B.v., Amsterdam.

Kurtzman, C.P. and Fell, J.W.. 1998. The yeasts: A taxonomic study. 4th ed. Elsevier Science Publisher B.V., Amsterdam.

Lutony, T.L. 1993. Tanaman sumber Pemanis. Penebar Swadaya, Jakarta.

Matsushima, R. and Baltz R.H. l986. Protoplas Fusion In Manual of Industrial Microbiology and Biotechnology. Demain, A.L. and Solomon, N.A. American Society for Microbiology. Washington DC.

Mayes, P.A., Daryl K.G., Victor W.R.and David W.M. l987. Biokimia Harper Terjemahan. Iyan Darmawan. Penerbit Buku Kedokteran EGC. Jakarta.

Moat, A.G. and J.W. Foster, l988. Microbial Physiology. John Wiley and Sons Inc., New York.

Nagy I.Z., C. Palagyi, Vagvolgyi and L. Fernczy. 1994. Genomic Comparison among Wild-type and Mutant Strains of P. rhodozyma. FEMS Microbiology. Lett. 123: 315-318

Panji, C. l985. Penuntun Praktikum Bioindustri. PAU IPB. Bogor.

Park, J.P and Yun J.W. 2001.Utilization of Chicory roots for Microbial Endoinulinase Production.Letters In Applied Microbiology. 2001 (33): 183 – 187

Park, S., Jeong, H.Y., Kim H.S., and Yang M.S. 2001. Enhanced Production of Aspergillus ficuum Endoinulinase in Saccharomyces cerevisiae By Using the SUC 2 Deletion Mutation. Enzyme and Microbial Technology Vol. 29

Pessoa A. and Vitolo M. 1999. Inulinase From Kluyveromyces marxianus: Culture Medium Composition and Enzyme Extraction. Journal Chemical Eng. Vol. 16.

Perkins,S. l984. Biotechnology: A New Industrial Revolision. Orbis Publishing. London.

Rouwenhorst, R.J.; Visser L.E. van Derbaan A.A. Scheffer W.A. and van Dijken J.P.. 1988. Production, Distribution and Kinetic Properties of Inulinase in Continous Culture of Kluyveromyces marxianus CBS 6556. Appl environ. Microbiol. 54(5): 1131 - 1137.

________; W.S. Ritmeester; W.A. Scheffer and J.P. van Dijken. 1990a. Localization of Innulinase and Invertase in Kluyveromyces sp.. Appl. Environ. Microbiol. 56 (11) : 3329 – 3336.

________; Hensing M. Verbakel J. Scheffer W.A. and van Dijken J.P.. 1990b. Structure and Properties of the Extracellular Inulinase of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 56 (11) : 3337 – 3345.

Rukmana, R. 2000. Dahlia: Prospek Agribisnis dan Teknik Budi Daya. Penerbit Kanisius. Jogyakarta.

Santiago, C.M. l982. Protoplast Fusion A New Tecnique for Genetic Manipulation and Breeding of Industrial Microorganisme. I.C. Biotech. 5: 435-440

Tjokroadikoesoemo, P.S. 1986. HFS dan Industri Ubi Kayu Lainnya. Penerbit PT. Gramedia, Jakarta.

Wijanarka. Arina, T.L dan Emi Y. 2001. Seleksi Khamir dan Optimasi Produksi Enzim Inulinase Dari Tanah Sekitar Umbi Ketela Rambat (Ipomoea batatas) di Daerah Bandungan Ambarawa. Penelitian BBI Dosen Muda 2001.

----------, Sri P. 2002. Isolasi Bakteri Termofilik Dalam Menghasilkan Enzim Inulinase Termostabil Dari Tanah Sekitar Umbi Dahlia (Dahlia sp) di Daerah Bandungan Ambarawa. Penelitian Dosen Muda, 2002.

Hermin, P.S., Wijanarka, Endang K dan Hermin P. 2003. Peningkatan Biosintesis Karotenoid pada Phaffia rhodozyma melalui Fusi Protoplas dalam Upaya Diversifikasi Pakan Buatan pada Sektor Akuakultur. Penelitian Dasar, 2003.

Wijanarka, Kusdiyantini E. dan Hermin P.S. 2004. Fusi Protoplas Interspesifik Kluveromyces marxianus dan Torulospora pretoriensis Authotonuos Untuk Produksi Enzim Inulinase Termostabil. Penelitian Dasar, 2004.

Wijanarka, Arina T.L. dan Hermin P.S. 2004. Fusi Protoplas Khamir Inulinolitik Kluveromyces marxianus dan Torulospora pretoriensis Isolat Lokal Serta Aplikasinya Pada HFS. Penelitian Terapan, 2005.

Xiao, R., M. Tanida and S. Takao. 1998. Inulinase from Crysosporium pannorum J. Ferment. Technol. 66 (5) : 244 – 248

_________; Tanida M. and Takao S. 1989. Purification and Some Properties of Endoinulinase from Crysosporium pannorum J. Ferment. Bioeng 67 (4): 244


Article Reads

Total: 853 Abstrak: 399 PDF: 454

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • Saat ini tidak ada refbacks.


 

Jumlah Kunjungan Harian

Satu bulan terakhir

Tampilkan laporan lengkap Google Analyticsdi sini
 

Laman ini dikelola oleh:
Bio Publisher
The Faculty of Biology Publishing

Laman ini dikelola oleh:
Penerbitan Fakultas Biologi
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telepon: +62-281-625865
Email: biologi@unsoed.ac.id

Laman ini menggunakan:
OJS | Open Journal System
Software pengelolaan jurnal ilmiah online. Versi yang digunakan adalah 2.4.8.0.

Metadata artikel terdaftar di:
Crossref
Agen resmi internasional pendaftaran Digital Object Identifier (DOI)

Artikel jurnal ini terindeks:

Daftar indeks »