N-Acyl Homoserine Lactones  sebagai Signal Quorum Sensing untuk Meningkatkan Efektifitas Bakteri Fosfat

Tamad Tamad

N-Acyl Homoserine Lactones sebagai Signal Quorum Sensing untuk Meningkatkan Efektifitas Bakteri Fosfat

Tamad Tamad⁽¹⁾

⁽¹⁾ Agroteknologi Fakultas Pertanian Universitas Jenderal Soedirman Jl. dr. Soeparno Karangwangkal Purwokerto 53123

Abstrak

Phosphate bacteria (PB) are able to release P-adsorption by soil. PB effectiveness in releasing adsorption P controled by quorum sensing (QS) signal. PB produces a QS signal as N-acyl homoserine lactones (N-HSL). The aim of this study are to determine the type of N-HSL as QS signal of PB (Pseudomonas trivialis, P. putida and P. fluorescens) and find the source of N-HSL from root extracts of some plants (rice, corn, bamboo, banana and peanuts). Analysis of N-HSL using HPLC (Hitachi UV-VIS detector L-2420), L-2200 autosampler (20 mL), L-2130 pump and column C OOF-4250-CO/10 µm LaChrom Ultra 18 (2 µm) 100 A 150 x 4.60 mm 10 m KPOW 490065-1 (Phenomenex), temperature 60° C, flow rate of 0.9 mL/minute and a gradient of 30-100 % in 1.0 minutes. Standard N-HSL is C4-HSL, 6, 8, 10, 12 homoserine lactones (Sigma-Aldrich, Germany) was dissolved in acetonitrile (Merch, India) with a concentration of 50 mM. P-dissolved by PB determined by staining NVM and a spectrophotometer at a wavelength of 413 nm. PB population is determined by the OD (optical density) at a wavelength of 600 nm. PB populations on medium Pikovskaya influenced by PB isolates, the type of P sources and duration of incubation. N-HSL generated by PB highest is Butanoyl (C₄) homoserine lactones. PB isolates 9 and Ca-phosphate sources produce N-HSL most. Root extract of rice, corn, bamboo, bananas and peanuts can be a source of N-HSL. Soluble phosphorus from medium Pikovskaya influenced by the type of PB isolates and source of P.

Kata Kunci

Autoinducer, P-adsorption, P-release, Root Exudate

Teks Lengkap:

PDF

Referensi

Balai Penelitian Tanah (BPT). 2005. Petunjuk Teknis: Analisis Kimia Tanah, Tanaman, Air dan Pupuk. Edisi Pertama. BPT, Balitbangtan Deptan, Bogor.

Camara M, Williams P, Hardman A. 2002. Controlling infection by tuning in and turning down the volume of bacteriall small-talk. Lancet Infectious Diseases 2: 667-676.

Hawkes CV, DeAngelis KM, Firestone MK. 2007. Root Interactions with Soil Microbial Communities and Processes. Pp. 1-30. In: Cordon, Z.G., and J.L. Whitbeck. (Eds.). The Rhizosphere: An Ecologival Perpective. Academic Press, New York. 210p.

Hosseinkhani B, Emtiazi G, Nahvi I. 2009. Analysis of phytase producing bacteria (Pseudomonas sp.) from poultry faeces and optimization of this enzyme production. African Journal of Biotechnology. 8(17): 4229-4232.

Li, X, Fekete A, Englmann M, Gotz C, Rothballer M, Frommberger M, Buddrus K, Fekete J, Cai C, Schroder P, Hartman A, Chen G, Kopplin PS. 2006. Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography. Journal of Chromatography 1134: 186-193.

Mehrvarz S, Chaichi MR. 2008. Effect of Phosphate Solubilizing Microorganisms and Phosphorus Chemical Fertilizer on Forage and Grain Quality of Barley (Hordeum vulgare L.). American-Eurasian J. Agric. & Environ. Sci. 3(6): 855-860.

Rani S, Kumar A, Malik AK, Koplin PA. 2011. Occurrence of N-Acyl Homoserine Lactones in Extracts of Bacteria Strain of Pseudomonas aeruginosa and in Sputum Sample Evaluated by Gas Chromatography–Mass Spectrometry. American Journal of Analytical Chemistry 2: 294-302.

Rashid, M., S. Khalil, N. Ayub, S. Alam, and F. Latif. 2004. Organic Acids Production and Phosphate Solubilization by Phosphate Solubilizing Microorganisms (PSM) Under in vitro Conditions. Pakistan Journal of Biological Sciences 7(2): 187-196.

Susilowati A, Wahyudi AT, Lestari Y, Suwanto A, Wiyono S. 2011. Potential Pseudomonas Isolated from Soybean Rhizosphere as Biocontrol againt Soilborne Phytophatogenic Fungi. Hayati J. Biosci. 18(2): 51-56.

Tamad. 2012. Mekanisme dan efektifitas bakteri fosfat dalam pelepasan fosfor di Andisol. [Disertasi], UGM, Yogyakarta. 196 hal.

Teplitski M, Mathesius U, Rumbaugh KP. 2011. Perception and Degradation of N-Acyl Homoserine Lactone Quorum Sensing Signals by Mammalian and Plant Cells. Chem. Rev. 111: 100-116.

Thiel T. 1999. Science in the Real World: Microbes in Action. Introduction to Bacterial. 1-8 p. Department of Biology, University of Missouri-St. Louis.

Tistama R, Widyastuti U, Sopandie D, Yokota A, Akashi K, Suharsono. 2012. Physiological and Biochemical Responses to Aluminium Stress in the Root of the Biodiesel Plant Jatrofa curcas L. Hayati J. Biosci. 19(1): 37-43.

Victoria DE, Reyes LL, Benitez AC. 2009. Use of 16S rRNA Gene for Characterization of Phosphate-Solubilizing Bacterial Associated with Corn. Rev. Fitotec. Mex. 32(1): 31-37.

Ward JP, King JR, Koerber AJ, Williams P, Croft JM, Sockett RE. 2001. Mathematical modelling of quorum sensing in bacteria. IMA Journal of Mathematics Applied in Medicine and Biology 18: 263-292.

Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill MEA. 2002. Structur Basis and Specificity of Acyl-Homoserine Lactone Signal Production in Bacteria Quorum Sensing. Molecular Cell 9: 685-694.