N-Acyl Homoserine Lactones sebagai Signal Quorum Sensing untuk Meningkatkan Efektifitas Bakteri Fosfat

Tamad Tamad (1)


(1) Agroteknologi Fakultas Pertanian Universitas Jenderal Soedirman Jl. dr. Soeparno Karangwangkal Purwokerto 53123


Abstrak


Phosphate bacteria (PB) are able to release P-adsorption by soil. PB  effectiveness in releasing adsorption P  controled by quorum sensing (QS) signal. PB  produces a QS signal  as N-acyl homoserine lactones (N-HSL). The aim of this study are to determine the type of N-HSL as QS signal of PB (Pseudomonas trivialis, P. putida and P. fluorescens) and find the source of N-HSL from root extracts of some plants (rice, corn, bamboo, banana and peanuts). Analysis of N-HSL using HPLC (Hitachi UV-VIS detector L-2420), L-2200 autosampler (20 mL), L-2130 pump and column C OOF-4250-CO/10 µm LaChrom Ultra 18 (2 µm) 100 A 150 x 4.60 mm 10 m KPOW 490065-1 (Phenomenex), temperature 60° C, flow rate of 0.9 mL/minute  and a gradient of 30-100 % in 1.0 minutes. Standard N-HSL  is C4-HSL, 6, 8, 10, 12 homoserine lactones (Sigma-Aldrich, Germany) was dissolved in acetonitrile (Merch, India) with a concentration of 50 mM. P-dissolved by PB determined by staining NVM and a spectrophotometer at a wavelength of 413 nm. PB population is determined by the OD (optical density) at a wavelength of 600 nm. PB  populations  on medium Pikovskaya influenced by PB isolates, the type of P sources and duration of incubation. N-HSL generated by PB highest is Butanoyl (C4) homoserine lactones. PB isolates 9 and Ca-phosphate sources produce N-HSL most. Root extract of rice, corn, bamboo, bananas and peanuts can be a source of   N-HSL. Soluble phosphorus from medium Pikovskaya influenced by the type of PB isolates and source of P.

Kata Kunci


Autoinducer, P-adsorption, P-release, Root Exudate

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Referensi


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