Determination of Iron and Zinc Absorption by Local Isolates of Saccharomyces cerevisiae to Produce Iron and Zinc in Organic Form

Hasim Munawar (1) , Eni Kusumaningtyas (2) , Agus Leeman (3) , David S. Silitonga (4) , Darmono Darmono (5)


(1) Toxicology Laboratory, Indonesian Research Centre for Veterinary Science (IRCVS)
(2) Mycology Laboratory, Indonesian Research Centre for Veterinary Science (IRCVS) Jalan R.E. Martadinata No 30 Bogor16114 Indoensia, tlp: 0251-8331048, fax: 0251-8336425
(3) Faculty of Pharmacy, Pancasila University Srengseng Sawah, Jagakarsa Jakarta 12640 Indonesia tel: +62 21 7864725 fax: +62 21 7864723
(4) Faculty of Pharmacy, Pancasila University Srengseng Sawah, Jagakarsa Jakarta 12640 Indonesia tel: +62 21 7864725 fax: +62 21 7864723
(5) Faculty of Pharmacy, Pancasila University Srengseng Sawah, Jagakarsa Jakarta 12640 Indonesia tel: +62 21 7864725 fax: +62 21 7864723



DOI: 10.20884/1.mib.2015.32.2.298


Abstrak


Iron (Fe) and Zinc (Zn) play important role in health both of live stock and human. Fe and Zn in organic form were claimed increasing their viabilities. They bind to certain amino acid formed as a product of microbial metabolism. The Amount Fe and Zn absorbed may indicated the Fe and Zn organic produced. The aim of the study is to determine the absorption of microelement of Fe and Zn by local isolates of Saccharomyces cerevisiae to produce Fe and Zn in organic forms. S. cerevisae BCC F0205, BCC F0206, and BCC F0214 were treated with Fe or Zn 10 ppm to obtain S. cerevisae which has the highest of total concentration of Fe and Zn. Selected isolate was then treated with Fe or Zn respectively 2.5, 5, 10 ppm and their combination. Fe and Zn absorbed by isolates were measured by Atomic Absorption Spectrophotometer (AAS). The results show that  S. cerevisae BCC F0205, BCC F0206, and BCC F0214 treated with 10 ppm Fe or Zn contained total concentration of Fe respectively 1.57, 3.07, 2.24 ppm  and total concentration of Zn respectively 2.34, 3.20, 3.13 ppm. Then, S. cerevisae BCC F0206 treated with 2.5, 5, 10 ppm Fe or Zn, absorbed Fe 1.45, 0.50, 0.94 ppm and Zn 0.73, 0.38, 0.53 ppm respectively. Furthermore, combination of Fe and Zn (2.5:2.5, 5:5, 10:10 ppm) produce absorption of Fe 3.10, 2.13, 3.67 ppm and Zn 0.11, 0.10, 0.28 ppm per gram S. cerevisae BCC F0206. Percentages of absorption by S. cerevisae are up to 100% for Fe and up to 47.20% for Zn. In conclusion, this study indicated that S. cerevisae BCC F0206 absorb Fe and Zn higher than BCC F0205 and BCC F0214 and there are antagonistic interactions found between Fe and Zn in this research.

Kata Kunci


Saccharomyces, yeast, iron, Zinc, Absorption

Teks Lengkap:

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Referensi


Ackland, M.L. and Michalczyk, A. 2006. Zinc Deficiency and its Inherited Disorders- A review. Genes & Nutrition 1 (1) : 41-50

Addai, K.N. 2014. Multi Nutrient Block Supplementation for Ruminants: Formulation and Manufacturing. Journal of Chemistry and Biochemistry 2 (1)

Aguilar, F., Charrondiere, U.R., Dusemund, B., Galtier, P., Gilbert, J., Gott, D.M., Grilli, S., Parent-Massin,Guertler, R., Kass, G.E.N., Koenig, J., Lambré, C., Larsen, J-C., Leblanc, J-C., Mortensen, A., Pratt, D I., Rietjens, I.M.C.M., Stankovic, I., Tobback, P., Verguieva, T., Woutersen, R. 2009. SCIENTIFIC OPINION : Inability to assess the safety of iron-enriched yeast as a source of iron, added for nutritional purposes to foods for particular nutritional uses and foods (including food supplements) intended for the general population, based on the supporting dossiers Scientific Statement of the Panel on Food Additives and NutrientSources added to Food (ANS). The EFSA Journal 1130 : 1-8

Arredondo, M., and Nunez, M.T. 2005. Review : Iron and copper metabolism. Molecular Aspects of Medicine 26 : 313–327

Blazejak, S. and Reinhard, W.D. 2004.Yeat Cell Biomass as a Potential Source of Magnesium Bioplexes-A Review. Pol. J. Food Nutr. Sci. (13/54) 3 : 223–232

Evans, G. W. & Johnson, E. C. 1980. Zinc absorption in rats fed a low-protein diet and a low protein diet supplemented with tryptophan or picolinic acid. J. Nutr. 110 : 1076-1080.

Fairweather-Tait, S. J., Payne, V.,Williams, C. M. 1984. The effect of iron supplements on pregnancy in rats given a low-zinc diet. Br. J. Nutr. 52 : 79-86.

Gordon, D. T. 1983. Interaction of iron and zinc on bioavailablity of each element in the rat. Fed. Proc. 42 : 1184

Gruden, N. & Momcovic, B. 1979. Zn transport in the duodenum and jejunum of rats fed milk enriched with iron. Nutr. Rep. Int. 19 : 483-489.

Hegoczki, 1994 in Gudreau H, Tompkins TA and Champagne CP. 2001. he distribution of iron-enriched cells of Sacharomyces cerevisiae. Act alimentaria 30(4): 355-361

Jang, Y.D., Kang, K.W., Piao, L.G., Jeong, T.S., Aauclair, E., Jonvel, S., D’Inca, RR., Kim, Y.Y. 2013. Effects of live yeast supplementation to gestation and lactation diets on reproductive performance, immunological parameters and milk composition in sows. Livestock Science. 152 : 167–173

King, J.C. 2000. Determinants of maternal zinc status during pregnancy. Am J Clin Nutr 71: 1334S–43S

King, M.W. 2006. Clinical aspect of iron metabolism. J. Med. Biochem. 15 (9) : 1- 4

Knutson, M., and Wessling-Resnick, M. 2003. Iron Metabolism in the Reticuloendothelial System. Critical Reviews in Biochemistry and Molecular Biology, 38(1):61–88

Korniewicz, D., Dobrzanski, Z., Chojnacka, K., Korniewicz, A., Kolacz, R. 2007. Effect of Dietary Yeasts Enriched with Cu, Fe and Mn on Digestibility of Main Nutrients and Absorption of Minerals by Growing Pigs. Am. J. Agril. & Biol. Sci., 2 (4): 267-275

Kumar, S., Pandey, A.K., Razzaque, W.A.D., and Dwivedi, D.K. 2011. Importance of micro minerals in reproductive performance of livestock. Veterinary World 4 (5) : 230-233

Kusumaningtyas, E., Widiastuti, R., Maryam, R. 2006a. Reduction of Aflatoxin B1 and M1 residues in duckc liver by giving culture of Saccharomyces cerevisiae and Rhizopus oligosporus. Prosiding: Seminar Nasional Teknologi Peternakan dan Veteriner . 790 -795

Kusumaningtyas, E., Widiastuti, R., Maryam, R. 2006b. Reduction of aflatoxin B1 in chicken feed by using Saccharomyces cerevisiae, Rhizopus oligosporus and their combination. Mycopathologia 162: 307–311

Maliken, B.D., Nelson, J.E., Klintworth, H.M., Beauchamp, M., Yeh, M.M., and Kowdley, K.V. 2013. Hepatic Reticuloendothelial System Cell Iron Deposition is Associated with Increased Apoptosis in Nonalcoholic Fatty Liver Disease. Hepatology 57(5): 1806–1813

Momcilovic, B. & Kello, D. 1977. The effect of milk enriched with iron on Zn absorption. Nutr. Rep. Int. 14 : 651-657.

Munawar, H. 2011. Comparison of Multi and Single Element Standards Used to Analyze Zinc(Zn) In Chicken and Beef. Proceeding : Seminar Nasional Teknologi Peternakan dan Veteriner.765 – 771

Nadadur, S.S., Srirama, K., and Mudipalli, A. 2008. Iron transport & homeostasis mechanisms: Their role in health and disease. Indian J Med Res 128 : 533-544

Pallauf, J. and fit Kirchgessner, M. 1974. Zinc status in depletion and repletion and its relation to vitamins and trace minerals. In: Trace Element Metabolism in Animals-2 (Hoekstra, W. G.,Suttie, J. W., Ganter, H. E. fit Mertz, W., eds.), University Park Press, Baltimore, MD.

Roy, S.K., Tomkins, A.M., Ara, G., Jolly, S.P., Khatun,W., Chowdhury, R., Chakrabarty, B. 2006. Impact of Zinc Deficiency on Vibrio cholerae Enterotoxin-stimulated Water and Electrolyte Transport in Animal Model. J Health Popul Nutr 24(1):42-47.

Sillerova, S., Blazena, L., Dana, U., Anezka P. Alena V, Ľuboš H. 2012. Preparation of Zinc Enriched Yeast (Saccharomyces cerevisiase) by Cultivation with defferent Zinc Salts. Journal of Microbiology, Biotechnology and Food Sciences. 1 : 689 - 695

Simm, C., Lahner, B., Salt, D., LeFurgey, A., Ingram, P., Yandell, B., Eide, D.J. 2007. Saccharomyces cerevisiae Vacuole in Zinc Storage and Intracellular Zinc Distribution. ASM, Eukaryotic Cell 6 (7) : 1166–1177

WHO, UNICEF and UNU. 2001. Iron Deficiency Anaemia Assessment, Prevention, and Control. A guide for programme managers .WORLD HEALTH ORGANIZATION. http://www.who.int/nutrition/publications/en/ida_assessment_prevention_control.pdf

Widhyari, S.D. 2012. The role and deficiency of zinc mineral in immune system. Wartazoa. 22 (3)


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