Keanekaragaman Genetik Tempuyung (Sonchus arvensis L.) Berdasarkan Marka Inter-Simple Sequence Repeats (ISSR)

Dyah Subositi (1) , Rohmat Mujahid (2)


(1) Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional
(2) Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional



DOI: 10.20884/1.mib.2019.36.2.828


Abstrak


Tempuyung (Sonchus arvensis L.) merupakan tanaman anggota familia Asteraceae yang banyak dimanfaatkan sebagai tumbuhan obat untuk menurunkan tekanan darah tinggi dan peluruh air seni. Tempuyung mudah dan banyak dijumpai di berbagai tempat di Indonesia. Keanekaragaman genetik tempuyung merupakan informasi dasar dalam rangka mendukung standarisasi tumbuhan obat. Penelitian ini bertujuan untuk melakukan karakterisasi genetik tempuyung menggunakan penanda molekuler ISSR (Inter Simple Sequence Repeats). Tiga belas aksesi tempuyung yang dikoleksi dari 8 lokasi digunakan sebagai sampel dan diamplifikasi DNAnya menggunakan 5 primer ISSR.Indeks similaritas dihitung berdasarkan indeks similaritas Dice. Analisis klaster menggunakan metode Unweighted Pair Group Method Using Arithmetic Mean (UPGMA) dilakukan untuk mengonstruksi dendogram. Hasil penelitian menunjukkan adanya variasi genetik antar aksesi tempuyung yang terbagi menjadi 3 klaster pada indeks similaritas 75,16%. Aksesi Purwokerto dan B2P2TOOT (4) mempunyai hubungan kemiripan yang terdekat pada indeks similaritas 90,90%. Penanda molekuler ISSR dapat digunakan dalam karakterisasi genetik antara aksesi tempuyung

Kata Kunci


karakterisasi genetik, tempuyung (Sonchus arvensis L.), ISSR

Teks Lengkap:

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Referensi


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