Aktivitas Protease, Amilase dan Lipase Digesti Ikan Medaka (Oryzias javanicus) yang Tertangkap di Segara Anakan Cilacap
Abstrak
Ikan medaka (Oryzias javanicus) yang berasal dari Segara Anakan Cilacap memiliki potensi sebagai biondikator perairan payau. Kajian karakter fisiologi O javanicus belum banyak dilakukan sehingga perlu ada informasi dasar untuk domestikasi. Tujuan penelitian ini untuk mengetahui perubahan aktivitas protease, amilase dan lipase digesti ikan medaka pada ukuran tubuh berbeda yang ditangkap dari Segara Anakan, Kabupaten Cilacap pada salinitas yang berbeda. Penelitian dilakukan dengan metode survey. Sampel ikan medaka diambil dari 3 stasiun dengan salinitas yang berbeda (10, 15 dan 20 ppt) dan diulang sebanyak 3 kali. Ikan medaka dikelompokkan menjadi 3 ukuran berbeda, yaitu (1) 0,063±0.01g (kecil), (2) 0,153±0.03 g (sedang), serta (3) 0,287±0.03 g (besar). Pengukuran aktivitas enzim dilakukan dengan metode spektrofotometri. Hasil penelitian menunjukkan bahwa ukuran tubuh dan salinitas yang berbeda berpengaruh nyata (P<0,05) terhadap aktivitas protease, amilase dan lipase digesti ikan medaka. Ikan medaka berukuran besar menghasilkan aktivitas enzim digesti lebih tinggi dibandingkan dengan ukuran tubuh kecil pada masing-masing salinitas. Namun pada salinitas 15 ppt, amilase dan lipase menunjukkan aktivitas yang seragam di antara ukuran tubuh berbeda. Semakin tinggi salinitas maka aktivitas protease, amilase dan lipase menjadi semakin rendah. Namun, pada salinitas 10-20 ppt menunjukkan aktivitas amilase (ukuran kecil dan sedang) dan lipase (ukuran kecil) yang sama. Simpulan dari hasil penelitian ini adalah aktivitas protease, amilase dan lipase organ digesti ikan medaka yang berasal dari Segara Anakan, Cilacap mengalami peningkatan seiring dengan semakin meningkatnya ukuran tubuh dan menurunnya salinitas lingkungan.
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