Ligninolytic Activity of Fungi Isolated from Empty Fruit Bunch of Oil Palm (Elaesis guineensis Jacq.)

N S Sepwin (1) , Erman Munir (2) , It Jamilah (3)


(1) Universitas Sampoerna
(2) Department of Biology, Faculty of Mathematics and Science, Universitas Sumatera Utara
(3) Department of Biology, Faculty of Mathematics and Science, Universitas Sumatera Utara


Abstrak


Lignin is a natural polymer and plays an important role as a compound of plant cell wall constituent. A study about the degradation of lignin in the environment has been receiving considerable attention because the complex structure and difficult to be degraded compared to the degradation of others plant cell wall constituent. A study to determine the activity of the ligninolytic enzyme (lignin peroxidase, manganese peroxidase and laccase) of fungi isolated from oil palm empty fruit bunch. This study has been done with a screening of ligninolytic activity using potato dextrose agar supplemented with tannic acid (0,1%), showed that two out of five fungal isolates have ligninolytic activity. The highest activity of lignin peroxidase was produced by SN2 isolatesi.e. 9.677U ml-1, whereas the highest activity of manganese peroxidase and laccase was produced by SN3isolates i.e.1.942 U ml-1 and 1.846 U m-1 respectively.


Kata Kunci


laccase, ligninolytic, lignin peroxidase, manganese peroxidase, oil palm empty fruit bunch

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Referensi


Artiningsih Artiningsih, T. 2006. Aktivitas Lignolitik Jenis Ganoderma pada Berbagai Sumber Karbon. Biodiversitas. 7(4): 307-311.

Badalyan, S., Szafranski., Hoegger, P., Gonzalez, N., Majcherczyk, A & Kues, U. 2011. New Armenian Wood-Associated Coprinoid Mushrooms: Coprinopsis strossmayeri and Coprinellus aff. Radians. Diversity. 3: 136-154.

Bonnen, A. M., Anton. L. H & Orth. A. B. 1994. Lignin-degrading enzymes of the commercial button mushroom, Agaricus bisporus. Appl. Environ.Microbiol. 60(3): 960-965.

Dey S., T.K. Maiti & B.C. Bhattacharyya. 1994. Production of Some Extracellular Enzymes by A Lignin Peroxidase-Producing Brown Rot Fungus, Polyporus ostreiformis, and Its Comparative Abilities for Lignin Degradation and Dye Decolorization. Appl. Environ. Microbiol. 60: 4216-4218.

Faizi, M., Shahriman., Majid, A., Shamsul., Basah., Cheng., Afendi, M., Zuradzman., Khairunizam & Hazry. 2016. An overview of the Oil Palm Empty Fruit Bunch (OPEFB) potential as reinforcing fibre in polymer composite for energy absorption applications. Matec Web of Conferences. 90: 1-9.

Isroi., Ishola, M., Millati, R., Syamsiah, S., Cahyanto, M., Niklasson, C & Taherzadeh, M. 2012. Structural Changes of Oil Palm Empty Fruit Bunch (OPEFB) after Fungal and Phosphoric Acid Pretreatment. Molecules. 17: 14997.

Kalra, K., Chaucan, R., Shavez, M & Sachveda, S. 2013. Isolation of Laccase Producing Trichoderma Spp. and Effect of PH and Temperature On Its Activity. International Journal of ChemTech Reasearch. 5(5): 2229-2235.

Kanwal H & Reddy M. 2011. Effect of carbon, nitrogen sources and inducers on ligninolytic enzyme production by Morchella crassipes. World. J. Microbiol. Biotechnol. 27: 687–691

Masalu, R. 2016. Ligninolytic Enzymes of the Fungus Isolated from Soil Contaminated with Cow Dung. Tanz. J. Sci. 42: 86-93.

Nishida, T., Y. Kashino, A. Mimura & Takahara. 1988. Lignin Biodegradation by Wood-Rotting Fungi I. Screening of Lignin-Degrading Fungi. Makuzai Gakkaishi. 34: 530-536.

Subowo. Y.B. & Corazon. 2010. Seleksi Jamur Tanah Pengurai Lignin dan PAH Dari Beberapa Lingkungan di Bali. Berita Biologi. 10(2): 227-233.

Sudiyani, Y., Sembiring. C. K., Hendarsyah. H & Alawiyah, S. 2010. Alkaline Pretreatment and Enzymatic Saccharification of Oil Palm Empty Fruit Bunch Fiber for Ethanol Production. Menara Perkebunan. 78(2): 70-74.

Wong, S. 2008. Structure and Action Mechanism of Ligninolitic Enzymes. Appl Biochem Biotechnol. 157: 174-209.

Xu, C., Ma, F & Zhang, X. 2009. Lignocellulose degradation and enzyme production by Irpex lacteus CD2 during solidstate fermentation of corn stover. J Biosci Bioeng. 108: 372-375


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