Screening Tumbuhan Alternatif Penghasil Xanthorrhizol Menggunakan Pendekatan In-Silico Berdasarkan Marker MatK

Diardy Shauman Rachmatan; Topik Hidayat

doi:10.20884/1.mib.2020.37.1.995

Screening Tumbuhan Alternatif Penghasil Xanthorrhizol Menggunakan Pendekatan In-Silico Berdasarkan Marker MatK

Diardy Shauman Rachmatan⁽¹⁾

, Topik Hidayat⁽²⁾

⁽¹⁾ Prodi Studi Biologi Fakultas Pendidikan Matematik dan Ilmu Pengetahuan Alam, Universitas Pendidikan Indonesia
⁽²⁾ Universitas Pendidikan Indonesia

DOI: 10.20884/1.mib.2020.37.1.995

Abstrak

Xanthorrhizol is a compound that has many uses such as anticancer, antimicrobial, anti-inflammatory, antioxidant, antihyperglycemic, antihypertensive, etc. However, this xanthorrhizol is only found in javanese turmeric (Curcuma zanthorrhiza) where this species only grows well in Indonesia. MatK from the GeneBank NCBI is used to find plants that are closely related to Curcuma zanthorrhiza. Multiple sequence alignment was done using ClustalX 1.83, and phylogenetic analysis was done using PAUP 4.0. The results of the reconstruction of the phylogenetic tree showed that white turmeric (Curcuma zedoaria) was the closest plant related to Curcuma zanthorrhiza. Curcuma zedoaria is a plant commonly found in tropical countries, such as India, Japan and Thailand. In Indonesia Curcuma zedoaria is known as Temu Putih. It is known that the chemical content of Temu Putih rhizome consists of curcuminoids, essential oils, and polysaccharides. Curcuminoid includes: curcumin, demethoxycurcumin, bisdemetoksikurkumin and 1,7-bis (4-hydroxyphenyl) -1,4,6-heptatrien-3-on. Curcuma zedoaria has rich essential oils: starch, curcumin, arabin, gums, etc. There is also more than ten sesquiterpene from Rhizome of Curcuma zedoaria such as furanodiene, furanodienone, zedorone, curzerenone, curzeone, germacrone, 13-hydroxy germacrone, dihydro-curdione, curcumenone and zedoaronediol. Based on that Curcuma zedoaria doesn’t produce xanthorrhizol naturally, but Curcuma zedoaria has a bioactive substance such as sesquiterpene and its closely related to Curcuma zanthorrhiza, genetic engineering can probably be used to produce xanthorrhizol.

Kata Kunci

MatK, , Xanthorrhizol, Zingiberaceae

Teks Lengkap:

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Referensi

Apavatjrut, P., Anuntalabhochai, S., Sirirugsa, P., and Alisi, C., 1999. Molecular Markers in the Identification of Some Early Flowering Curcuma L. (Zingiberaceae) species. Ann. Bot. 84, pp. 529-534.

Hargono D. 1996. Sekelumit Mengenai Obat Nabati dan Sistem Imunitas. Cermin Dunia Kedokteran 108: 6-10.

Islam, M.R., Ahamed, R., Akbar, M.A., Alam, K. and Food, U.S., 2010. In Vitro Antimicrobial Activities of Four Medicinally Important Plants in Bangladesh. (January).

Jang, M. K., Sohn, D. H., Ryu, J. H., 2001, A Curcuminoid and Sesquiterpenes as Inhibitor of Macrophage TNF-a Release from Curcuma zedoaria, Planta Med, 67: 550-552

Lobo, R., Prabhu, K.S., Corporation, H.M., Shirwaikar, A. and Shirwaikar, A., 2009. Curcuma zedoaria Rosc, ( white turmeric ): A review of its chemical, pharmacological and ethnomedicinal properties. (February).

Maciel N. and Criley R.A. 2003. Morphology growth and flowering behavior of Curcuma zedoaria. Acta Hort. (ISHS) 624: 111-116.

Makabe H et al. 2006. Antiinflammatory sesquiterpenes from Curcuma zedoaria. Nat Prod Res. 2006; 20: 680–686.

Matsuda H., Ninomiya K., Morikawa T. and Yoshikawa M. 1998. Inhibitory effect and action mechanism of sesquiterp-enes from Zedoaria rhizome on D-galactosamine/lipopoly-saccharide- induced liver injury. Bioorg. Med. Chem. Lett. 8: 339-344.

Nadkarni KM. 1999. Indian Materia Medica, 3rd edn. Mumbai, India: Popular Prakashan Private Limited

Oon, S. F., Nallappan, M., Tee, T. T., Shohaimi, S., Kassim, N. K., Sa’ariwijaya, M. S. F., & Cheah, Y. H. 2015. Xanthorrhizol: A review of its pharmacological activities and anticancer properties. Cancer Cell International, 15(1). https://doi.org/10.1186/s12935-015-0255-4

Purseglove J.W., Brown E.G., Green C.L. and Robbins S.R.J. 1981. Spices. Vol. 2. Chapter 9, Tropical Agriculture Series, Longman, London and New York

Rukayadi, Y., Lee, K., Han, S., Yong, D. and Hwang, J. K. 2009. In vitro activities of panduratin A against clinical Staphylococcus strains. Antimicrobial Agents and Chemotherapy 53(10): 4529-4532.

Sasaki Y., Fushimi H., Cao H., Cai S.Q. and Komatsu K. 2002. Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication. Biol. Pharm. Bull. 25: 1593-1599.

Sembiring BB, Ma’mun, & Ginting EI. 2006. Pengaruh kehalusan bahan dan ekstraksi terhadap mutu ekstrak temulawak (Curcuma xanthorrhizo Roxb.) Buletin Balitro. 17(2):53- 58

Sirirugsa P. 1999. Thai Zingiberaceae: Species diversity and their uses. International Conference on Biodiversity and Bi-oresources: Conservation and Utilization. Phuket, Thailand.

Syu, W. J., Shen, C. C., Don, M. J., Ou, J. C., Lee, G. H., Sun, C. M., 1998, Cytotoxicity of Curcuminoids and Some Novel Compounds from Curcuma zedoaria. Journal of Natural Product, 61(12): 1532-1534

The Plant List, 2013. Version 1.1. Published on the Internet; http://www.theplantlist.org/ [Accessed 1 January].

www.ncbi.nlm.nih.gov/GenBank

Yoshioka T., Fujii E., Endo M., Wada K., Tokunaga Y., Shiba N., Hohsho H. and Muraki T. 1998. Antiinflammatory potency of dehydrocurdione a zedoary-derived sesquiterpene. Inflamm. Res. 47: 467-481.

Yusuf M. 2000. Genetic diversity of the family Zingiberaceae Bangladesh. Ph.D. Thesis. Department of Botany, Jahangirnagar University, Dhaka, Bangladesh.

Záveská, E., Fér, T., Šída, O., Krak, K., Marhold, K., & Leong-škorničková, J. 2016. Phylogeny of Curcuma (Zingiberaceae) based on plastid and nuclear sequences: Proposal of the new subgenus Ecomata Author (s): Eliška Záveská , Tomáš Fér , Otakar Šída , Karol Krak , Karol Marhold and Jana Published by : International Association for , 61(4), 747–763.