FORTIFIED FEED OF AVOCADO (Persea americana) LEAF EXTRACT FOR SEPTICEMIA MOTILE AEROMONAD DISEASE PREVENTION IN CATFISH

Ardiansyah Kurniawan (1) , Dara Novera Jumita (2) , eva prasetiyono (3)


(1) Bangka Belitung University
(2) 
(3) Universitas Bangka Belitung


Abstract


Disease caused by the bacterium A. hyrophila known as MAS (Motile Aeromonad Septicaemia) decreases survival and growth in Catfish. Phytopharmaca is a potential solution to replace antibiotics. One of the phytopharmaca which has the potential to be effective, inexpensive, safe for humans, and environmentally friendly is avocado (Persea americana) with active compounds such as alkaloids, flavonoids, saponins, and tannins. The potential for prevention of bacterial diseases with avocado leaf extract on the clinical symptoms of fish in MAS disease needs to be scientifically proven to be applicable to aquaculture economically and environmentally sustainable. This study uses an experimental method with several stages, namely testing LD50, in vitro and in vivo tests. The LD50 concentration of A. hydrophila is at a density of 102 cfu/ml. The filtrate of avocado leaf maceration showed that a clear zone diameter is equivalent to penicillin in the in-vitro test. Fortification of avocado leaf extract on feed is quite effective in preventing MAS disease in the in-vivo test. The fastest of MAS disease clinical symptoms recovery is at a dose of 100 ppt and 300 ppt on the 7th day. The highest Survival Rate (SR) was found at a dose of 100 pp (100%) which was equivalent to negative control and followed by a dose of 200 ppt (73.33%), 300 ppt (66.67%) and positive control (46.67%).


Keywords


Aeromonas hydrophila, Persea americana, Fortification of feed, immunostimulants

References


Apriyati, Nilda. 2014. Isolasi dan Karakterisasi Senyawa Alkaloid dari Daun Alpukat. Skripsi. Jurusan Pendidikan Kimia. Universitas Negeri Gorontalo

Bricknell I. dan R. A. Dalmo, 2005. The use of immunostimulants in fish larval aquaculture. Fish & Shellfish Immunology. Vol. 19. p 457-472

Dewi, Endra. 2006. Efek Anti Imflamasi Ekstrak Etanol Daun Senggani (Melastoma polyanthum BI) Pada Mencit Betina. Skripsi. Fakultas Farmasi. Universitas Sanata Dharma. Yogyakarta

Gannam A.L dan R.M. Schrock. 2008. Immunostimulants in Fish Diets. Journal of Applied Aquaculture. Volume 9. Issue 4. p 53-89

Gede D., E. Suryanto, dan F. Wehantouw. 2009. Potensi Daun Alpukat (Persea americana Mill) Sebagai Sumber Antioksidan Alami. Chem-Prog. Vol. 2 No. 1.

Holt JG, NR Krieg, PHA Sneath and JT Staley. 1994. Bergey’s Manual of Determinative Bacteriology. The Williams and Wilkins. Baltimore, Marylan. USA.

Husnia, Ketty. 2016. Sehat Tanpa Obat Dengan Alpukat. Rapha Publishing. Yogyakarta.

Ismarani. 2012. Potensi Senyawa Tannin Dalam Menunjang Produksi Ramah Lingkungan. Jurnal Agribisnis dan Pengembangan Wilayah. Vol. 3 No. 2.

Ismiyati N., dan Trilestari. 2014. Pengembangan Formulasi Masker Ekstrak Air Daun Alpukat (Persea americana Mill) Sebagai Antibakteri Staphylococcus aureus Untuk Pengobatan Jerawat. Pharmaciana. Vol. 4 No. 1. p 45-52

Komarudin O. dan J. Slembrouck. 2005. Petunjuk Teknis Pembenihan Ikan Patin Indonesia (Pangasius djambal). Bab VIII Manajemen Kesehatan Ikan. IRD (Lembaga Penelitian Prancis untuk Pembangunan). Jakarta. Indonesia.

Kristinawati. 2010. Pengaruh Ekstrak Daun Alpukat (Persea americana Mill.) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Putih Jantan Dengan Metode Uji Toleransi Glukosa. Skripsi. Fakultas Farmasi. Unika Widya Mandala Surabaya.

Kurniawan, Ardiansyah. 2011. Seleksi Bakteri Antagonis Larva Patin Siam (Pangasius hypopthalmus) Terhadap Aeromonas hydrophila. Akuatik - Jurnal Sumberdaya Perairan. Vol. 5. Nomor 1. p 1-4

Laporan Pemantauan Daerah Sebar Hama Dan Penyakit Ikan Karantina. 2016. Stasiun Karantina Ikan Pengendali Mutu Dan Keamnana Hasil Perikanan Kelas 1 Pangkalpinang. Kementrian Kelautan Dan Perikanan.

Lucia, F. Charyadie, S. Adi, dan R.P. Sari. 2014. Daya Hambat Ekstrak Daun Alpukat (Persea Americana, Mill) Terhadap Pertumbuhan Enterococcus faecalis. Denta Jurnal Kedokteran Gigi. Vol 8. No. 1

Mehana E.E., A.H. Rahmani, dan S.M Aly. 2015. Immunostimulants and Fish Culture: An Overview. Annual Research & Review in Biology. Vol 5. No. 6. p 477-489

Melangngi L., M.S. Sangi, dan J.E Paendong.. 2012. Penentuan Kandungan Tanin dan Uji Aktivitas Antioksidan Ekstrak Biji Buah Alpukat (Persea americana Mill). Jurnal Mipa Unsrat Online. Vol. 1 No.1 p 5-10.

Plumb, Jonh A. 1994. Health Maintenance And Principal Microbial Diseases Of Cultured Fishes. CRC Press. Boca Raton. Florida.

Sangi M., R.J.Runtuwene, E.I Simbala, dan M.A. Veronica. 2008. Analisis Fitofarmaka Tumbuhan Obat Di Kabupaten Minahasa Utara. Chem-Prog. Vol.1. No.1

Sartika,Y. 2011. Efektivitas Fitofarmaka dalam Pakan untuk Pencegahan Infeksi Bakteri Aeromonas hydrophila pada Ikan Lele Dumbo (Clarias sp). Skripsi. Dapartemen Budidaya Perairan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.

Srivastava P. K. dan A. K. Pandey. 2015. Role Of Immunostimulants In Immune Responses Of Fish And Shellfish. Biochem. Cell. Arch. Vol. 15, No. 1, pp. 47-73

Wahjuningrum D., E.H. Solokhah, T. Budiardi, dan M. Setiawati. 2010. Pencegahan Infeksi Aeromonas hyrophila Pada Ikan Lele Dumbo (Clarias sp.) dengan Campuran Meniran (Phyllanthus niruri) dan Bawang Putih (Allium sativum) dalam Pakan. Jurnal Akuakultur Indonesia. Vol 9 No.2 p 93-103.


Article Reads

Total: 2098 Abstract: 2098

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BOKEP INDO

BACKLINK BOKEP INDO BY SEO JEMBUT

 

Daily Visit

The last two months

View complete Google Analytics report here
 

This website is maintained by:
Bio Publisher
The Faculty of Biology Publishing

Faculty of Biology
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865
Email: biologi@unsoed.ac.id

T his website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version 2.4.8.0.

All article content metadata are registered to:
Crossref
An official nonprofit  Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list  »