The Application of Two Steps Culture in Agarwood, Aquilaria malaccensis, In Vitro Culture Improves Microshoots Induction and Development

Vindi Ambara Putri (1) , Sugiyono Sugiyono (2) , Lucky Prayoga (3) , Rendie Prasetyo (4) , Sharon Hilary (5)


(1) Faculty of Biology, Universitas Jenderal Soedirman
(2) Faculty of Biology, Universitas Jenderal Soedirman
(3) Faculty of Biology, Universitas Jenderal Soedirman
(4) Faculty of Biology, Universitas Jenderal Soedirman
(5) Faculty of Biology, Universitas Jenderal Soedirman


Abstract


Agarwood (Aquilaria malaccensis) is an important species with high economic value and has many benefits, which led to an overexploitation in its natural habitat.  An effort to both provides sufficient seedling for agarwood cultivation and conservation is therefore much needed. This study has been carried out with a view to determine the effect of media types and BAP concentrations in two steps cultivation system on agarwood microshoot induction. This was a two stage-experiments i.e., microshoots induction and optimizing shoot development.  The research results showed that the interaction between different media type and BAP concentrations had no significant effect on agarwood microshoot induction.  Subsequent culture on MS media without any BAP addition showed that explant derived from MS medium solidified with 2.5 gr. L-1 Phytagel produced 2.36 ± 0.48 shoots/explant and 3.69 ± 1.16 leaves/explant.  In addition, explant derived from culture on MS medium supplemented with 4 µM BAP produced 2.28 ± 0.61 shoots/explant. This is for the first time the application of two steps culture system for Agarwood (Aquilaria malaccensis) has been deployed and how the habituation phenomenon is handled.


Full Text:

PDF

References


Ahmadian M, Babaei A, Shokri S, Hessami S. 2017. Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid culture. J Genet Eng Biotechnol. 15(2):309-315.
https://doi.org/10.1016/j.jgeb.2017.07.005

Arti LT, Mukarlina. 2017. Multiplikasi Anggrek Bulan ( Dendrobium sp .) Dengan Penambahan Ekstrak Taoge Dan Benzyl Amino Purine (BAP) Secara InVitro. Protobiont. 6(3):278-282.

Ashraf MF, Aziz MA, Kemat N, Ismail I. 2014. Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of chlorophytum borivilianum sant. & fernandez. Electron J Biotechnol. 17(6):275-279.
https://doi.org/10.1016/j.ejbt.2014.08.004

Feng J, Shi Y, Yang S, Zuo J. 2017. Cytokinins. Horm Metab Signal Plants.:77-106.
https://doi.org/10.1016/B978-0-12-811562-6.00003-7

George EF. 1993. Plant propagation by Tissue Culture Part I. England: The Technology Exegetics.

George EF, Sherington PD. 1984. Plant Propagation by Tissue Culture. England: The Technology Exegetic.

Hafizh LT, Yenni, Siregar AS, Maghfoer MD. 2018. Induksi Tunas Eksplan Batang Kultur Maristem Stroberi (Fragaria chiloensis) dengan Teknik Perendaman TDZ (Thidiazuron) pada Kombinasi Media MS dan ZPT. J Produksi Tanam. 6(7):1442-1450.

Istikomah N. 2012. Upaya Mencari Bahan Pengganti Agar pada Mikropropagasi Kentang Kultivar Granola. Universitas Jendral Soedirman.

Kamínek M, Motyka V, Vaňková R. 1997. Regulation of cytokinin content in plant cells. Physiol Plant. 101(4):689-700.
https://doi.org/10.1034/j.1399-3054.1997.1010404.x

Karyadi AK, Ahmad B. 2008. Pengaruh Komposisi Media Dasar, Penambahan BAP, Dan Pikloram Terhadap Induksi Tunas Bawang Merah. J Hortik. 18(1):85239. doi:10.21082/jhort.v18n1.2008.p.

Maxiselly Y, Sari RA, Ariyanti M. 2020. Stimulasi Pertumbuhan Kina Belum Menghasilkan (TBM) dengan Aplikasi Konsentrasi BAP setelah Fase Pembentukan Batang. J Paspalum. 8(2):78-74.
https://doi.org/10.35138/paspalum.v8i2.180

Nam YJ, Tran LSP, Kojima M, Sakakibara H, Nishiyama R, Shin R. 2012. Regulatory Roles of Cytokinins and Cytokinin Signaling in Response to Potassium Deficiency in Arabidopsis. PLoS One. 7(10).
https://doi.org/10.1371/journal.pone.0047797

Nath PC, Nath AJ, Sileshi GW, Das AK. 2020. Stand structure and functional attributes of agarwood (Aquilaria malaccensis Lam.) based smallholder farms in Northeast India. Trees, For People. 2(July):100027.
https://doi.org/10.1016/j.tfp.2020.100027

Pierik RLM. 1982. In vitro Culture of Higher Plants.

Prasetyo R, Sugiyono, Prayoga L. 2020. Induksi tunas mikro pisang kultivar ambon nangka ( Musa sp .) secara in vitro. Vigor J Ilmu Pertan dan Subtrop. 5(2):45-50.
https://doi.org/10.31002/vigor.v5i2.3044

Reinert J, Yeoman MM. 1982. Plant Cell and Tissue Culture: A Laboratory Manual. http://link.springer.com/10.1007/978-3-642-81784-7.
https://doi.org/10.1007/978-3-642-81784-7

Schaller GE, Street IH, Kieber JJ. 2014. Cytokinin and the cell cycle. Curr Opin Plant Biol. 21:7-15.
https://doi.org/10.1016/j.pbi.2014.05.015

Sembiring SLN, Siregar LAM, Kardhinata EH. 2018. Keberhasilan Terbentuknya Tunas Mikro Anggrek (Cattleya Trianae Lindl & Rchb.Fil.) Dalam Beberapa Komposisi Medium. J Agroekoteknologi. 6(1, Januari):113-117. doi:10.32734/jaet.v6i1,Januari.17229.

Sugiyono. 1993. Pengaruh Hormon 2,4-D dan BAP Terhadap Multiplikasi Kalus Purwoceng (Pimpinella pruatjan Molkenb) Pada Kultur Aseptis. Universitas Jendral Soedirman.

Suthar RK, Habibi N, Purohit SD. 2011. Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrataRoxb. Indian J Biotechnol. 10:224-227.

Trivedi MK, Branton A, Trivendi D, Nayak G, Bairwa K, Jana S. 2015. Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media. Cell Biol. 3(4):50.
https://doi.org/10.11648/j.cb.20150304.11

Vardja R, Vardja T. 2001. the Effect of Cytokinin Type and Concentration and the Number of Subcultures on the Multiplication Rate of Some Decorative Plants. Proc Est Acad Sci Biol Ecol. 50(1):22.
https://doi.org/10.3176/biol.ecol.2001.1.03

Wang M, Zheng Q, Shen Q, Guo S. 2013. The critical role of potassium in plant stress response. Int J Mol Sci. 14(4):7370-7390.
https://doi.org/10.3390/ijms14047370

Wardatutthoyyibah, Wulandari RS, Herlina D. 2015. Penambahan auksin dan sitokinin terhadap pertumbuhan tunas dan akar gaharu (Aquilaria malaccensis Lamk) secara in vitro. J Hutan Lestari. 3(1):43-50.


Article Reads

Total: 781 Abstract: 346 PDF: 435

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

Daily Visit

The last two months

View complete Google Analytics report here
 

This website is maintained by:
Bio Publisher
The Faculty of Biology Publishing

Faculty of Biology
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865
Email: biologi@unsoed.ac.id

T his website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version 2.4.8.0.

All article content metadata are registered to:
Crossref
An official nonprofit  Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list  »