UPAYA MEMACU PERTUMBUHAN TUNAS MIKRO KENTANG KULTIVAR GRANOLA DENGAN JENIS DAN KONSENTRASI SITOKININ BERBEDA

Adinda RN Pratama (1) , Sugiyono Sugiyono (2) , Lucky Prayoga (3) , Ali Husni (4)


(1) Fakultas Biologi, Universitas Jenderal Soedirman
(2) Fakultas Biologi, Universitas Jenderal Soedirman
(3) Fakultas Biologi, Universitas Jenderal Soedirman
(4) 


Abstract


Micro-shoot growth is the first step in the potato microtuber formation. The main factors were known controlling microtuber formation including the media type, the concentration of sucrose, the type and concentration of plant growth regulators, temperature, and photoperiodicity. This research aimed to determine the influence of the t of cytokinin and its concentration on the micro-shoot formation of Granola cultivar of potato (Solanum tuberosum L.), as well as to determine the best cytokinin type and its concentration on micro-shoot formation of Granola cultivar potato (Solanum tuberosum L.). The research was experimental in a split-plot design. The main plot was the type of cytokinin (BAP and Kinetin), and the subplot was cytokinin concentration at five levels of concentrations, i.e., 0, 5, 10, 15 and 20 µM. This study replicated each treatment three times which resulted in obtaining 30 experimental units. The data obtained were then analyzed using an analysis of variance, followed by honest significant difference test at 95 and 99% levels of confidence. The results showed that the formation of micro-shoot of Granola cultivar of potato was controlled by the type and concentration of cytokinin used. The addition of 5‒15 μM of BAP was found to be the best treatments to stimulate micro-shoot formation of Granola cultivar of potato.


Keywords


Potato; Granola Cultivar; Microtuber; Microshoot; BAP; Kinetin

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References


Alix MJ, Savvides S, Blakef J, Herrmanin R, Horinung R. 2001. Effects of Lumination Source, Culture Ventilation and Sukrosa on Potato (Solanum Tuberosum) Microtuber Production Under Short Days. Ann. Appl. Biol. 139:175–8. https://doi.org/10.1111/j.1744-7348.2001.tb00394.x

Altindal D, Karadogan T. 2010. The Effect of Carbon Sources on In Vitro Microtuberization of Potato (Solanum Tuberosum L.). Turkish Journal of Field Crops. 15(1):7–11.

Ambarwati AD. 1987. Induksi Kalus dan Differensiasi pada Kultur Jaringan Gnetum gnemon L. Fakultas Biologi Universitas Gadjah Mada. Yogyakarta.

Ansyar M, Ala A, Baharuddin dan Elkawakib S. 2009. Potensi Ekstrak Daun Gamal dan Filtrat Cendawan Diplodia spp. sebagai Penginduksi Umbi Mikro Kentang secara In Vitro. J. Sains & Teknologi, Vol.9 No.2 : 97 – 105I SSN 1411 – 4674

Aryakia E, Hamidoghli Y. 2010. Comparison of kinetin and 6-banzyl amino purine effect on in vitro microtuberization of two cultivars of potato (Solanum tuberosum L.). American-Eurasian J. Agric. & Environ. Sci. 8(6):710–714.

Asandhi AA. 1996. Tumpangsari kentang pada lahan sawah di dataran medium. J. Hort.6(1):23-8.

Asandhi AA, Gunadi N. 1989. Syarat Tumbuh Tanaman Kentang dalam Asandhi, A.A., S. Sastrosiswojo; Suhardi; Z. Abidin dan Subhan (Eds). Balai Penelitian Holtikultura. Lembang. Hal. 22 – 29.

Badoni A, Chauhan JS. 2010. Potato seed production of cultivar kufri himalini. Invitro. Stem Cell 1(1):7–10.

Bertell G, Eliason L. 1992. Cytokinin Effect on Root Growth and Possible Interaction with Ethylene and Indole-3-acetic Acid. Physiologia Plantarum. The Scandinavian Society for Plant Physiology Copenhagen. 84(2):255–261. https://doi.org/10.1111/j.1399-3054.1992.tb04662.x

Bostan H, Demirct E. 2004. Obtaining PVX, PVY and PLRV-free micro tuber from Granola and Caspar potato (Solanum tuberosum L.) Cultivars. Pakistan J. of Biological Sci.7(7):1135–1139. https://doi.org/10.3923/pjbs.2004.1135.1139

Buchory A, Karjadi AK. 2007. Pengaruh Konsentrasi BAP dan Sumber Karbohidrat Gula terhadap Induksi Umbi Mikro Kentang. Balai Penelitian Tanaman Sayuran. J. Agrivigor 6(3):197-205.

El-Sawy A, Bekheet S, Aly UI. 2007. Morphological and Molecular Characterization of Potato Microtubers Production on Coumarin Inducing Medium. International Journal of Agriculture & Biology. 9(5):675–680.

Fatima B, Usman M, Ahmad I, Khan IA. 2005. Effect of Explant and Sukrosa on Microtuber Induction in Potato Cultivars. International Journal of Agriculture & Biology. 7(1):63–66.

Garner N, Blake J. 1989. The Induction and Development of Potato Microtubers In vitroon Media Free of Growth Regulating Substances. Annals of Botany. 63:663¬–674.

George EF, Sherrington PD. 1984. Plant propagation by tissue culture. Exegetics Ltd. p:39–71

Gopal J, Minocha JL, Dhaliwal HS. 1998. Microtuberization in Potato (Solanum tuberosum L.). Plant Cell Reports. 17:794–798. https://doi.org/10.1007/s002990050485

Gunarto A. 2007. Prospek Agribisnis Kentang G4 Sertifikat di Kabupaten Sukabumi. Pusat Pengkajian dan Penerapan Teknik Budidaya Pertanian.

Heylen C, Vendrig JC, Onckelen HV. 1991. The Accumulation and Metabolism of Plant Growth Regulator During Organogenesis. Physiologia Plantarnum. 83(4):578–584. https://doi.org/10.1111/j.1399-3054.1991.tb02471.x

Hoque ME. 2010. In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics Journal. 3(1):7–11.

Hussey G, Stacey NT. 1981. In vitro propagation of potato (Solanunitubevosuni L). Annals of Botany. 48;787–796. https://doi.org/10.1093/oxfordjournals.aob.a086186

Imani AA, Qhrmanzadeh R, Azimi J, Janpoor J. 2010. The Effect of Various Concentrations of 6-Benzylaminopurine (BAP) and Sukrosa on In Vitro Potato (Solanum Tuberosum L.) Microtuber Iduction. American-Eurasion J. Agric. & Environ. Sci. 8(4):457‒459.

Krishnamoorthy HN. 1981. Plant Growth Substances including Application in Agriculture. Tata McGrawHill Publ. Co. Ltd. New Delhi. 214p.

Kusumaningrum IS. 2007. Evaluasi Pertubuhan In vitro dan Produksi Umbi Mikro Beberapa Klon Kentang (Solanum tuberosum L.) Hasil Persilangan Kultivar Atlantik dan Granola [Skripsi]. Program Studi Hortikultura Fakultas Pertanian Institut Pertanian Bogor.

Manurung LY, Santi. 2007. Pengaruh Auksin (2,4-D) dan Sitokinin (BAP) dalam Kultur in Vitro Buah Makasar (Brucea javanica [L.] Merr.) [Skripsi]. Departemen Konservasi Sumberdaya Hutan dan Ekowisata Fakultas Kehutanan Institut Pertanian Bogor.

Mellor FC, Stace-Smith R. 1987. Virus-free potatoes through meristem culture. Biotechnology in Agriculture and Forestry.Vol.3 Potato. Springer–Verlag Berlin Heidelberg.

Moeini MJ, Armin M, Asgharipour MR, Yazdi SK. 2011. Effects of Different Plant Growth Regulators and Potting Mixes on Micro-propagation and Mini-tuberization of Potato Plantlets. Advances in Environmental Biology. 5(4):631–638.

Nistor, A., G. Campeanu, N. Atanasiu, N. Chiru, D. Karácsonyi, 2010. Influence of Genotype on Microtuber Production. Not. Bot. Hort. Agrobot. Cluj 38 (1): 209 – 212.

Novak, P.J. 1, J. Zadina, V. Horackova and I. Maskora. 1980. The effect of growth regulators on meristem tip development and in vitro multiplication of Solanum tuberosum L. Plants. Potato Res. 23: 155 – 156. https://doi.org/10.1007/BF02356266

Piao, X. C., D. Chakrabarty, E. J. Hahn, K. Y. Paek, 2003. A simple method for mass production of potato microtubers using a bioreactor system. Current Science 84 (8): 1129 – 1132.

Pierik RL. 1987. IN VITRO CULTURE OF HIGHER PLANTS AS A TOOL IN THE PROPAGATION OF HORTICULTURAL CROPS. InInternational Symposium on Propagation of Ornamental Plants 226. pp.25-40).

Rafique, T., M. J. Jaskani, H. Raza, M. Abbas, 2004. In vitro Studies on Microtuber Induction in Potato. International Journal of Agriculture & Biology, 06(2): 375 – 377

Rukmana, 1997. Budidaya Kentang dan Pasca panen. Kanisius, Yogyakarta.

Rusiman. 2008. Potato Plant (Tanaman Kentang). http://www. rusiman.bpdas-pemalijratun.net/ index.php? option=com: tanaman-kentang. Diakses 11 November 2011.

Salisbury FB, Ross CW. 1994. Plant Physiology. Wadsworth Publishing, Belmont.

Santi A, Kusumo S. 1996. Komposisi media tumbuh yang cocok untuk perbanyakan Secara In Vitro Bromelia (Tillandsia punctulata L.). Jurnal Hortikultura.5(5):94-8.

Soelarso, R. B. 1997. Budidaya Kentang Bebas Penyakit. Kanisius. Yogyakarta.

Sugiyono, 1993. Pengaruh Pemberian Hormon 2,4-D dan BAP Terhadap Multiplikasi Kalus Purwoceng (Pimpinella Pruatjan Molkenb.) pada Kultur Aseptis. [Skripsi]. Fakultas Biologi Universitas Jenderal Soedirman.

Thurton, H.D. 2001. Origin, history and importance of the potato in Stevenson, W.R. (eds). Compendium of Potato Disease. 2th ed. The American Phytopathological Soc. New York.

Uijtewall BA. 1987. The Production and Evaluation of Monophodial Potatoes (2n=X=12) for Breeding Research on Cell and Plant Level. Grafisch Bedrijf Ponsen & Looijen. Wageningen. P12.

Ummah K, Purwito A. 2009. Budidaya Tanaman Kentang (Solanum tuberosum L.) dengan Aspek Khusus Pembibitan di Hikmah Farm, Pangalengan, Bandung, Jawa Barat. Makalah Seminar. Departemen Agronomi dan Hortikultura Fakultas Pertanian Institut Pertanian Bogor.

Uranbey S. 2005. Comparison of Kinetin and 6-Benzyladenine (BA) on in vitro Microtuberization of Potato under Short Days Conditions. Tarım Bilimleri Dergisi. J. Agric. Sci. 15(1):39–41.

Uranbey S, Parmaksız İ, Sancak C, Çöçü S, Özcan S. 2004. Temperature and gelling agent effects on in vitro microtuberization of potato (Solanum tuberosum L.). Biotechnology & Biotechnological Equipment. 18(2):89-94. https://doi.org/10.1080/13102818.2004.10817092

Vander Zaag P, Wei H. 1991. Potato (Solanum spp). Apical cutting production and their agronomic potential. Asian Potato J. 20(1):17-22.

Wattimena GA. 2000. Pengembangan Propagul Kentang Bermutu dari Kultivar Kentang Unggul dalam Mendukung Peningkatan Produksi Kentang di Indonesia. Orasi Ilmiah Guru Besar Tetap Ilmu Hortikultura Fakultas Pertanian Institut Pertanian Bogor.

Wattimena GA, Gunawan LW, Mattjik NA, Syamsudin E, Armini NM, Ernawati A. 1992. Perbanyakan Tanaman dalam Bioteknologi Tanaman. PAU Bioteknologi Institut Pertanian Bogor. Dirjen Dikti Dept. P&K.

Zakaria M, Hossain MM, Mian MAK, Hossain T, Uddin MZ. 2008. In Vitro Tuberization of Potato Influenced by Benzyl Adenine and Chloro Choline Chloride. Bangladesh J. Agril. Res. 33(3):419-425. https://doi.org/10.3329/bjar.v33i3.1601

Zulkarnain. 2009. Kultur Jaringan Tanaman Solusi Perbanyakan Tanaman Budi Daya. PT. Bumi Aksara, Jakarta.

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