Handa Muliasari (1) , Candra Dwipayana Hamdin (2) , Muhsinul Ihsan (3)

(1) Program Studi Farmasi, Universitas Mataram
(2) Program Studi Farmasi, Universitas Mataram
(3) Jurusan Biologi, FTK, UIN Mataram


Brucea javanica (L) Merr is a local plant used traditionally for diabetic medication in Lombok Island. The B. javanica seed has been recognized to have an antidiabetic effect, while the impact of the leave extract was understudied. The purpose of this research was to determine the effect of B. javanica leaves infusion on the blood glucose level of alloxan-induced diabetic rats. B. javanica leaves extract were applied by soaking leaves into hot water (95°C) to obtain the extract concentration of 5% (w/v). This experiment provides four treatments to rats within 15 days with five replicates for each treatment. The treatments for rats were K1 a positive control, K2 a negative control, K3 a healthy control, and P1 treated with leave infused water. The diabetic condition for K1, K2, and P1 was induced with an intravenous injection of 125 mg/kgBW alloxan monohydrate to the rats. After stable diabetic condition, K1 rats were treated with Glibenclamide (0,25 mg/kgBW), K2 rats were untreated, P1 rats were administered with leaves infused water of B. javanica (L) Merr 3 ml/day. K3 rats were uninduced and untreated. The hypoglycemic effect was observed by estimating the fasting blood glucose level of the rats every three days. After 15 days of treatments, P1 rats blood glucose level reduced by the 13,30%, while K1 rats 22,04%. The decrease in blood glucose level of both K1 and P1 rats have not reached the average blood glucose level of healthy rats. The result of the statistical analysis showed the infused extract effect was not significantly differed compared to positive control (P < 0.05).

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Ablat A, Mohamad J, Awang K, Shilpi JA, Arya A. 2014. Evaluation of Antidiabetic and Antioxidant Properties of Brucea javanica Seed. Hindawi Publishing Corporation, The Scientific World Journal, volume 2014, https://doi.org/10.1155/2014/786130

Chackrewarthy S, Tahbrew MI. 2014. Hypoglycaemic and Hypolipidaemic Effects of an Ethylacetate Fraction of Artocarpus heterophyllus Leaves. Chapter 12. http://dx.doi.org/10.5772/52538 https://doi.org/10.5772/52538

Chang CLT, Lin Y, Bartolome AP, Chen YC, Chiu SC, Yang WC. 2013. Review Article Herbal Therapies for Type 2 Diabetes Mellitus: Chemistry, Biology, and Potential Application of Selected Plants and Compounds. Evidence-Based Complementary and Alternative Medicine. Vol. 2013. Article ID 378657. Hindawi Publishing Corporation. 33 p.

Coman C, Rugina OD, Socaciu C. 2012. Plants and Natural Compounds with Antidiabetic Action. Notulae Botanicae Horti AgrobotaniciCluj-Napoca. 40(1):314-325, Print ISSN 0255-965X; Electronic 1842-4309.

Eid HM, Haddad PS. 2014. Review Article: Mechanisms of Action of Indigenous Antidiabetic Plants from the Boreal Forest of Northeastern Canada. Journal of Advances in Endocrinology. 2014:1-11. https://doi.org/10.1155/2014/272968

Issa IA, Bule MH. 2015. Hypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan Induced Diabetic Swiss Albino Mice. Evidence-Based Complementary and Alternative Medicine. Volume 2015. Article ID 752486. http://dx.doi.org/10.1155/2015/752486. 5 p. https://doi.org/10.1155/2015/752486

Hout S, Chea A, Bun S, Elias R, Gasquet M, Timon-David P, Balansard G, Azas N. 2006. Screening of selected indigenous plants of Cambodia for antiplasmodial activity. Journal of Ethnopharmacology. 107(1):12-18.

Medscape. 2017. Glyburide: Dosing and uses. https://reference.medscape.com.

Nasution R, Barus T, Nasution P, Saidi N. 2014. Isolation and Structure Elucidation of Steroid from Leaves of Artocarpus camansi (Kulu) as Antidiabetic. International Journal of PharmTech Research CODEN (USA). 6(4):1279-1285.

Njagi JM, Ngugi MP, Kibiti CM, Ngeranwa J, Njue W, Gathumbi P, Njagi E. 2015. Hypoglycemic effect of Helichrysum odoratissimum in alloxan induced diabetic mice. The Journal of Phytopharmacology (JPHYTO). 4(1):30-33.

Njogu SM, Arika WM, Nyamai DW, Ngugi MP, Machocho AK, Ngeranwa JJN, Njagi ENM. 2016. Hypoglycemic effect of aqueous and ethyl acetate leaf extract of Maytenus putterkloides in alloxan induced diabetic mice. Journal of Diabetes & Metabolism. 7(6):685.

Noorshahida A, Wong TW, Choo CY. 2009. Hypoglicemic effect of quassionoids from Brucea javanica (L) Merr (Simaroubaceae) seeds. Journal of Ethnopharmacology. 124(3):586-91.

Okuyama E, Gao LH, Yamazaki M. 1990. Studies on pharmacologically active principles from Indonesia crude drugs. III. Toxic components from Brucea javanica (L) Merr. Yakugaku Zasshi. 110(11):834-8.

Osadebel PO, Odoh EU, Uzor PF. 2014. Review: The search for new hypoglycemic agents from plants. African Journal of Pharmacy Fand Pharmacology. 8(11):292-303.

Papich MG. 2016. Saunders Handbook of Veterinary Drugs (Fourth Edition). P. 359-360. http://doi.org/10.1016/B978-0-323-24485-5-00283-7.

Prameswari OM, Widjanarko SB. 2014. Uji Efek Ekstrak Air Daun Pandan Wangi Terhadap Penurunan Kadar Glukosa Darah Dan Histopatologi Tikus Diabetes Mellitus. Jurnal Pangan dan Agroindustri. 2(2):16-27.

Sakaki T, Yoshimura S, Tsuyuki T, Takahasi T, Honda T, Nakanishi T. 1986. Structures of Yadanziosides K, M, N, and O, New Quassinoid Glycosides from Brucea javanica (L.) MERR. Bulletin of the Chemical Society of Japan. 59(11):3541-3546. https://doi.org/10.1246/bcsj.59.3541

Walvekar MV, Potphode ND, Desai SS, Deshmukh VM. 2016. Histological Studies on Islets of Langerhans of Pancreas in Diabetic Mice after Curcumin Administration. International Journal of Pharmaceutical and Clinical Research. 8(9):1314-1318.

World Health Organization. 2000. Global Report On Diabetes. Available from: http://apps.who.int/iris/bitstream/10665/204871/1/9789241565257_eng.pdf

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