Hafsan Hafsan (1) , Nurjannah Nurjannah (2) , Cut Muthiadin (3) , Isna Rasdianah Aziz (4) , Ahyar Ahmad (5) , Laily Agustina (6) , Asmuddin Nasir (7)

(1) Universitas Islam Negeri Alauddin Makassar
(2) Universitas Islam Negeri Alauddin Makassar
(3) Universitas Islam Negeri Alauddin Makassar
(4) Universitas Islam Negeri Alauddin Makassar
(5) Universitas Hasanuddin
(6) Universitas Hasanuddin
(7) Universitas Hasanuddin


This study aimed to determine the optimum temperature and pH of the phytase activity produced by isolate of thermophilic bacteria from Sulili hot springs in the district Pinrang south sulawesi. This is a descriptive research, that measure of phytase activity by treated the variation of temperature and pH. The various of temperature has eight levels ie 20; 30; 40; 50; 60; 70; 80; and 90oC, the pH treatments consists of eight levels ie 2; 3; 4; 5; 6; 7; 8 and 9. Phytase activity was determined by converting the Na-phytate absorbance values produced by phytase enzyme against Na-phytate standard curve. One unit of phytate enzyme activity is defined as the amount of enzyme capable of hydrolysed Na-phytate and produce a peptide which is equivalent to one mole of tyrosine per minute under the conditions of analysis. The results of studies have shown that there is a significant effect of temperature on the phytase activity of Bacillus coagulans, thermophilic bacteria from Sulili hot springs, whereas no significant effect of pH treatment. Optimum phytase activity of B. coagulans are at 60°C and pH 7 ie 6.11 units/ mL.


Bacillus coagulans; phytase; Sulili hot springs; temperatures and pH; thermophilic bacteria


Angelis MD, Gallo G, Corbo MR, McSweeney PLH, Faccia M, Giovine M, Gobetti M. 2003. Phytase activity in sourdough lactic acid bacteria: purification and characterization of a phytase from Lactobacillus sanfranciscensis CB1. International Journal of Food Microbiology 87(3):259-270. doi: 10.1016/S0168-1605(03)00072-2.

Bhavsar K, Kumarb VR, Khirea JM. 2012. Downstream Processing of Extracellular Phytase from Aspergillus niger: Chromatography Process vs. Aqueous Two Phase Extraction for its Simultaneous Partitioning and Purification. Process Biochem. 7(47):1066–1072

Blaabjerg K, Thomassen AM, Poulsen HD. 2015. Microbial phytase addition resulted in a greater increase in phosphorus digestibility in dry-fed compared with liquid-fed non-heat-treated wheat– barley–maize diets for pigs. Anim. 9(2):243–248.

Choi YM, Suh HJ, Kim JMJ. 2001. Purification and Properties of Extracellular Phytase from Bacillus sp. KHU-10. Protein Chem 20: 287. doi: 10.1023/A:1010945416862.

El-Toukhy NMK, Youssef AS, Mikhail MGM. 2013. Isolation, purification and characterization of phytase from Bacillus subtilis. African J. Biotechnol. 12:2957-2967.

Hafsan, Irwan I, Agustina L, Ahmad A, Natsir A. 2017. Isolation and characterization of phytase-producing thermophilic bacteria from sulili hot springs in South Sulawesi. SCIRJ. 5(12):16-19.

Hasyimuddin, Djide MN, Samawi MF. 2016. Isolasi Bakteri Pendegradasi Minyak Solar Dari Perairan Teluk Pare-Pare. Biogenesis 4(1):41-46. doi: 10.24252/bio.v4i1.1119.

Huang H, Shao N, Wang Y, Luo H, Yang P, Zhou Z, Zhan Z, Yao B. 2009. A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. Appl Microbiol Biotechnol 83: 249. doi: 10.1007/s00253-008-1835-1.

Kusumadjaja S, Piliang WG, Ketaren PP. 2009. Suplementasi enzim pemecah serat dan phytase terhadap performans. JITV. 1(14):40-46.

Lan GQ, Abdullah N, Jalaludin S, Ho YW. 2002. Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle. Journal of Applied Microbiology 93(4):668-674. doi: 10.1046/j.1365-2672.2002.01727.x.

Madigan MT, Martinko J, Parker J. 2004. Brock Biology of Microorganisms 10th Edition. NJ: Pearson Prentice Hall.

Mandviwala TN and Khire JM. 2000. Production of high activity thermostable phytase from thermotolerant Aspergillus niger in solid state fermentation. J Ind Microbiol Biotech 24: 237. doi: 10.1038/sj.jim.2900811.

Pawiroharsono S. 2003. Microbial enzyme and their application in industry. Proceeding of Seminar Industri Enzim dan Bioteknologi. Malang: CIEB.

Tomschy A, Brugger R, Lehmann M, Svendsen A, Vogel K, Kostrewa D, Lassen SF, Burger D, Kronenberger A, van Loon APGM, Pasamontes L, Wyss M. 2002. Engineering of Phytase for Improved Activity at Low pH. Appl. Environ. Microbiol 68(4):1907-1913. doi: 10.1128/AEM.68.4.1907-1913.2002.

Vats P and Banerjee UC. 2004. Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): an overview. Enzyme and Microbial Technology 35(1):3-14. doi: 10.1016/j.enzmictec.2004.03.010

Vielle C and Zeikus GJ. 2001. Hyperthermophilic enzymes: sources, uses, and molecular mechanism, for thermostability. Microbiol Mol Biol Rev. 7(65):14-18.

Article Reads

Total: 402 Abstract: 402

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Daily Visit

The last two months

View complete Google Analytics report here

This website is maintained by:
Bio Publisher
The Faculty of Biology Publishing

Faculty of Biology
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865

T his website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version

All article content metadata are registered to:
An official nonprofit  Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list  »