ANALYSIS OF GENETIC VARIATION MUTANT SOYBEAN ( Glycine max . L.MERR) GAMMA RAYS AND EMS (ETHYL METHANE SULFONATE) INDUCTION THROUGH ISSR MOLECULAR MARKERS

Evika Sandi Savitri

ANALYSIS OF GENETIC VARIATION MUTANT SOYBEAN (Glycine max. L.MERR) GAMMA RAYS AND EMS (ETHYL METHANE SULFONATE) INDUCTION THROUGH ISSR MOLECULAR MARKERS

Evika Sandi Savitri⁽¹⁾

⁽¹⁾

Abstract

Mutation induction in soybeans was carried out to develop high genetic diversity as a basis for plant breeding to get superior varieties. Mutation induction can be done using physically mutagen gamma rays and chemically using EMS mutagens. This study aims to determine the effectiveness of chemical mutagen (EMS) and physical mutagen (gamma rays) in the induction of genetic diversity in soybeans. Mutation detection can be done with molecular markers to characterize plant genetic diversity. In this study, ISSR molecular markers were used consisting of 4 primers. The method of mutation induction used EMS and gamma rays treatment. The results of the primary PCR amplification of ISSR2, ISSR3 and UBC888 showed 100% polymorphism. The treatment of mutation induction using EMS chemical agents showed a higher degree of polymorphism compared to gamma rays treatment

References

Bornet, B. and Branchard, M., 2001. Nonanchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant molecular biology reporter, 19(3), pp.209-215.

Darmono TW. 2011. Analisis keragaman genetic Ganoderma spp . yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA ( RAPD ). Menara Perkebunan. 79(1):6–14.

Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 11-15.

Godwin, I.D., Aitken, E.A. and Smith, L.W., 1997. Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis, 18(9), pp.1524-1528.

Goyal, S., Kozgar, M. I., & Khan, S. (2012). Effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combinations in Vigna mungo (L.) Hepper. Biorem. Biodiv. Bioavail, 6, 113-115.

Khan, M.H. and Tyagi, S.D., 2013. A review on induced mutagenesis in soybean. Journal of Cereals and Oilseeds, 4(2), pp.19-25.

Kumar, D. S., Nepolean, T., & Gopalan, A. (2003). Effectiveness and efficiency of the mutagens gamma rays and ethyl methane sulfonate on umabean (Phaseolus lunatus L.). Indian Journal of Agricultural Research, 37(2), 115-119.

Lazar I., Lazar I. Gel Analyzer. 2010: Freeware 1D gel electrophoresis image analysis software. 2010. Available online: http://www.gelanalyzer.com.

Mangaiyarkarasi, R., Girija, M., Gnanamurthy, S., & Don, C. L. G. (2014). Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonate in Catharanthus roseus. Int. J. Curr. Microbiol. Appl. Sci, 3, 881-889.

Nagaoka, T. and Ogihara, Y., 1997. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and applied genetics, 94(5), pp.597-602.

Novita L. 2013. Analisis genetik karakter morfoagronomi jarak pagar hasil pemuliaan berbasis pendekatan kuantitatif dan molekuler. Tesis. 1–50.

Pavadai, P., Girija, M. and Dhanavel, D., 2010. Effect of gamma rays on some yield parameters and protein content of soybean in M2, M3 and M4 generation. Journal of Experimental Sciences, 1(6).

Prevost, A. and Wilkinson, M.J., 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98(1), pp.107-112.

Prevost, A., & Wilkinson, M. J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and applied Genetics, 98(1), 107-112.

Roldàn-Ruiz, I., Dendauw, J., Van Bockstaele, E., Depicker, A., & De Loose, M. A. F. L. P. (2000). AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular breeding, 6(2), 125-134.

Shah, T. M., Atta, B. M., Mirza, J. I., & Haq, M. A. (2011). Induced genetic variability in chickpea (Cicer arietinum L.) III. Frequency of morphological mutations. Pak. J. Bot, 43(4), 2039-2043.

Soengas, P., Velasco, P., Padilla, G., Ordás, A., & Cartea, M. E. (2006). Genetic relationships among Brassica napus crops based on SSR markers. HortScience, 41(5), 1195-1199.

Varshney, R. K., Nayak, S. N., Jayashree, B., Eshwar, K., Upadhyaya, H. D., & Hoisington, D. A. (2007). Development of cost-effective SNP assays for chickpea genome analysis and breeding. Journal of SAT Agricultural Research, 3(1), 1-3.

Zietkiewicz, E., Rafalski, A. and Labuda, D., 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2), pp.176-183.

Article Reads

Total: 1732 Abstract: 1682

Refbacks

There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Daily Visit

The last two months

View complete Google Analytics report here

This website is maintained by:
Bio Publisher
The Faculty of Biology Publishing

Faculty of Biology
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865
Email: biologi@unsoed.ac.id

T his website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version 2.4.8.0.

All article content metadata are registered to:
Crossref
An official nonprofit Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list »

Username
Password
Remember me