Natasya Nur Musrifa (1)

(1) Gadjah Mada University


Indonesia with an area of tropical waters 5.4 billion km2 depend on the largest source of foreign exchange earnings derived from prawns. Freshwater prawns of the genus Macrobrachium shown a wide distribution and high economic value. Bali has potential for high freshwater prawns diversity with an area 10,000 Ha of cultivation. Pakerisan river is one of Bali’s rivers with high population, so affected prawns ecosystem in habitat. The study was conducted to determine the diversity of freshwater prawn species belonging to genus Macrobrachium based on morphological identification and molecular analysis with 16S mitochondrial gene from the downstream of Pakerisan river. Sampling was administered by Gianyar Research Team in March 2015. Initial identification and determination of morphological characters was done. Afterwards, identification of prawns molecular characters using primers 16Sar and 16Sbr were analyzed by DNAStar software, MESQUITE, DNA Sequence Polymorphism 5.10, and MEGA 7.0.21. The results based on morphological identification of individual CR1 and CR2 that was: second pereiopod had longer carpus than merus and chela, epistome split into 2 round anterior lobes, rostral formula: 3+5/3, and 2 pairs of spines on parallel telson. Supported by molecular identification DNA fragment length sequencing were 561bp. BLAST analysis similarity 99%, average genetic distance 0.24%, and bootstrap value 100% in reconstruction with Neighbor-Joining phylogeny tree showed monophyletic group with seven samples sequence Macrobrachium lancesteri from NCBI GenBank. So morphological and molecular identification was declared CR1 and CR2 individuals included to species Macrobrachium lanchesteri.



Identification, prawns, Macrobrachium lanchesteri, Pakerisan, Bali


Agustina M. 2016. Distribusi dan Preferensi Habitat Udang dan Kepiting Air Tawar (Crustacea: Decapoda) di Danau Laut Tawar Aceh Tengah, Indonesia (Tesis). Bogor. Institut Pertanian Bogor. Hal: 19.

Butler TH. 1980. Shrimps of the Pacific coast of Canada. Ottawa.Canada. Bulletin 202, Department of Fisheries and Oceans. P: 280.

Cai Y and Ng PKL. 2002. The Freshwater Palaemonid Prawns (Crustacea: Decapoda: Caridea) of Myanmar. Hydrobiologia 487:59-83.

Cai Y, Naiyanetr P, Ng PKL. 2004. The Freshwater prawns of the genus Macrobrachium Bate, 1868, of Thailand (Crustacea: Decapoda: Palaemonidae). Journal of Natural History 38: 581-649.

Chong SSC, Khoo HW. 1988. The Identity of Macrobachium lanchesteri (De Man, 1911) (Decapoda, Palaemonidae) from Peninsular Malaysia and Singapore, and a Description of its First Zoea. Crustaceana 54(2): 195-206.

Chong, SSC, Khoo HW, and Ng PKL. 1987. Presence of the Japanese Freshwater Prawn Macrobrachium Nipponense (De Haan, 1849) (Decapoda: Caridea: Palaemonidae) In Singapore. Zool. Med., Leiden, 61(22): 313-317.

Daryanto, Hamidah A, dan Kartika WD. 2015. Keanekaragaman jenis udang air tawar di danau teluk Kota Jambi. Biospecies. (8)1: 13-19.

De Grave S, Fransen CHJM. 2011. Carideorum catalogus: the recent species of the dendrobranchiate, stenopodidean, procarididean and caridean shrimps (Crustacea: Decapoda). Zool. Meded., 85: 195-588.

Grant WAS, Bowen BW. 1998. Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation. J. Hered. 89: 415-426.

Hadi S, Susandarini R, Epilurahman R, Yudha DS, Asti HA, dan Purnomo. 2016. Keanekaragaman Floran dan Fauna Daerah Aliran Sungai Pakerisan Kabupaten Gianyar. Yogyakarta. UGM Press. Hal: 29-31.

Han T, Lee W, Lee S, Park LG, Park H. 2016. Reassessment of Species Diversity of the Subfamily Denticollinae (Coleoptera: Elateridae) through DNA Barcoding. PLoS ONE 11(2): e0148602. doi:10.1371/journal.pone.0148602.

Hernawati RT. 2013. Hubungan Kekerabatan Crustacea (Decapoda) di Sungai Cijalu Kecamatan Majenang Kabupaten Cilacap (Skripsi).Purwokerto. Universitas Jendral Soedirman. Hal: 23-26.

Holthuis LB. 1950. The Decapoda of the Siboga Expedition Part X. The Palaemonidae.Collected by the Siboga and Snellius Expeditions with Remarks on the Other Species I. Subfamily Palaemoninae. Netherland: Leiden E.J Brill.pp :4-6,12,98-101.

IUCN. 2013. diakses pada tanggal 18 Agustus 2018.

Johnson DS. 1967. Biology of Potentially Valuable Fresh-water Prawns with Special Reference to the Riceland Prawn Cryphiops (Macrobrachium) lanchesteri (de Man). FAO Fisheries Report 57(2): 77-587.

Lanchester WF. 1902. On the crustaceans collected during the "Skeat" Expedition to the Malay Peninsula, together with a note on the genus Actaeopsis, Part I: Brachyura, Stomatopoda, and Macrura. Proceedings of the Zoological Society of London, 1901: 534-574, Pls.33-34.

Murphy NP, Austin CM. 2005. Phylogenetic relationships of the globally distributed freshwater prawn genus Macrobrachium (Crustacea: Decapoda: Palaemonidae): biogeography, taxonomy and the convergent evolution of abbreviated larval development. Zool. Scr. 34: 187-197.

Nei M. 2001. Genetic distance. Academic Press. doi: 10.006/rwgn.2001.0532

New MB. 2002. Farming Freshwater Prawns. A manual for the culture of the giant river prawn (Macrobrachium rosenbergii). Marlow, United Kingdom.Food and Agriculture Organization of the United Nations. pp :9,15,20.

Osawa S, Su Z, Imura Y. 2004. Molecular Phylogeny and Evolution of Carabid Graound Beetles. Hong Kong. Springer-Verlag Tokyo: SNP Best-set Typesetter Ltd. P: 176.

Pereira G. 1997. A cladistic analysis of the freshwater shrimps of the Family Palaemonidae (Crustacea, Decapoda, Caridea). Acta Biol. Venez. 17: 1-69.

Saputri K. 2017. Peluang Kendala Ekspor Udang Indonesia ke Pasar Jepang. Jurnal Ilmu Hubungan Internasional 5(4):1179-1194.

Short JW. 2004. A revision of Australian river prawns, Macrobrachium (Crustacea: Decapoda: Palaemonidae). Hydrobiologia.Vol 525:1-100.

Supriadi A. 2012.Keanekaragaman Jenis Udang Air Tawar di Sungai-sungai yang Berasal dari Gunung Salak (Tesis). Bogor. Institut Pertanian Bogor.Hal: 3.

Wowor D, Cai Y, Ng PKL. 2004. Crustacea: Decapoda, Caridea. In: C. M. Yule & H. S. Yong (Eds.), Freshwater Invertebrates of the Malaysian Region. Academy of Sciences Malaysia, Kuala Lumpur Pp. 337-357.

Wowor D, Cai Y, Ng PKL. 2012. Crustacea: Decapoda, Caridea. Freshwater Invertebrates of the Malaysian Region. Pp: 337-357.

Wowor D, Muthu V, Meier R, Balke M, Cai Y, Ng PKL. 2009. Evolution of life history traits in asian freshwater prawns of genus Macrobrachium (Custacea: Decapoda: Palaemonidae) based on multilocus molecular phylogenetic analysis. Mol Phylogenetic and Evol 52: 340-350.

Yang L, Tan Z, Wang D, Xue L, Guan M, Huang T, Li R. 2014. Spesies Identification trough mitochondrial rRNA genetic analysis. Scientific Report. Vol 4 : 4089.Doi:10.1038/srep0408.

Article Reads

Total: 431 Abstract: 431

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


This website is maintained by:
Bio Publisher
The Faculty of Biology Publishing

Faculty of Biology
Universitas Jenderal Soedirman
Jalan dr. Suparno 63 Grendeng
Purwokerto 53122

Telephone: +62-281-625865

This website uses:
OJS | Open Journal System
A free journal management and publishing system that has been developed by the PKP (Public Knowledge Project) version

All article content metadata are registered to:
An official nonprofit Registration Agency of the International Digital Object Identifier (DOI) Foundation.

Articles in this journal are indexed by:

Complete index list »