AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT Anguilla bicolor McClelland)

Mohamad Taufik (1) , Hana Hana (2) , Untung Susilo (3)


(1) Fakultas Biologi, Universitas Jenderal Soedirman
(2) Fakultas Biologi, Universitas Jenderal Soedirman
(3) Fakultas Biologi, Universitas Jenderal Soedirman


Abstract


This study was experimental, conducted using a Completely Randomized Design (CRD) with 3 x 2 factorial design, and four replicates have been carried out to evaluate the protease and amylase activities of Anguilla bicolor McClelland. A total of 71 individuals divided into three weight groups were used in this study.  The first group with an average weight of 41.25 ± 0.898 g consisted of 51 eels,  the second with an average weight of 319.8 ± 4.666 g composed of 14 eels, and the third with a mean weight of 569.5 ± 9.150 g consisted of 6 eels. The results showed the protease activity differed significantly based on eel size and intestinal segment (P ˂ 0.05). This research recorded the highest protease activity was in eels within the smallest weight group (41.25 ± 0.898 g). This study also revealed the protease activity in the anterior intestine was higher than the posterior in all size of eels. The amylase activity did not differ significantly (P>0.05) by eel size and intestinal segment. This study concluded the protein digestion capacity of smaller eels was higher than larger eels, and the protein digestion capacity was greater in the anterior intestine than the posterior intestine. The carbohydrate absorption capacity in eel was not affected by the variety of fish size which indicates no change in the feed category.


Keywords


protease; amylase; intestine; Anguilla bicolor

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References


Abowei JFN, Ekubo AT. 2011. Some principles and requirements in fish nutrition. British Journal of Pharmacology and Toxicology. 2(4):163-178.

Al-Gadri SF, Susilo U, Priyanto S. 2014. Aktivitas protease dan amilase pada hepatopankreas dan intestine ikan nilem (Osteochilus Hasselti C.V.). Scripta Biologica. 1(1):43-48. https://doi.org/10.20884/1.sb.2014.1.1.24

Al-Tameemi R, Aldubaikul A, Salman NA. 2010. Comparative study of α-amylase activity in three cyprinid species of different feeding habits from Southern Iraq. Turkish Journal of Fisheries and Aquatic Sciences. 10:411-414. https://doi.org/10.4194/trjfas.2010.0315

Caruso G, Denaro MG, Genovese L. 2008. Temporal changes in digestive enzyme activities in the gastrointestinal tract of European Eel (Anguilla anguilla) (Linneo 1758) following feeding. Marine and Freshwater Behaviour and Physiology. 41(4):215-228. https://doi.org/10.1080/10236240802492931

Caruso G, Denaro, MG, Genovese L. 2009. Digestive enzymes in some teleost species of interest for mediterranean aquaculture. The Open Fish Science Journal. 2(1):74-86. https://doi.org/10.2174/1874401X00902010074

Chakrabarti R, Rathore RM, Mittal P, Kumar S. 2006. Functional changes in digestive enzymes and characterization of proteases of silver carp (♂) and bighead carp (♀) hybrid, during early ontogeny. Aquaculture. 253:694–702. https://doi.org/10.1016/j.aquaculture.2005.08.018

Chiu ST, Pan BS. 2002. Digestive protease activities of juvenile and adult eel (Anguilla japonica) fed with floating feed. Aquaculture. 205(1):141–156. https://doi.org/10.1016/S0044-8486(01)00669-X

Furne M, Hidalgo MC, Lopez A, Garcia GM, Morales AE, Domezain A, Domezaine J, Sanz A. 2005. Digestive enzyme activities in adriatic sturgeon (Acipenser naccarii) and rainbow trout (Onchorynchus mykiss): a comparative study. Aquaculture. 250:391-398. https://doi.org/10.1016/j.aquaculture.2005.05.017

Gawlicka A, Brigitte P, Horn MH, Neil R, Ingergjerd O, Ole JT. 2000. Activity of digestive enzyme in yolk-sac larvae of atlantic halibut (Hippoglossus hippoglossus): Indication of readiness for first feeding. Aquaculture. 184:303-314. https://doi.org/10.1016/S0044-8486(99)00322-1

Handayani S, Zairin MJ, Mokoginta I, Bintang M, Sudrajat AO. 2008. Perubahan enzim-enzim pencernaan pada ikan gurame (Osphronemus gouramy) sebagai respon terhadap pakan yang mengandung kadar protein dan karbohidrat yang berbeda. Aquacultura Indonesiana. 9(1):25–29.

Hidalgo MC, Urea E, Sanz A. 1999. Comparative study of digestive enzymes in fish with different nutritional habits : proteolytic and amylase activities. Aquaculture. 170:267-283. https://doi.org/10.1016/S0044-8486(98)00413-X

Jiang J, Feng L, Tang L, Liu Y, Jiang W, Zhou X. 2015. Growth rate, body composition, digestive enzymes and transaminase activities, and plasma ammonia concentration of different weight Jian carp (Cyprinus carpio var. Jian). Animal Nutrition. 1(4):373-377. https://doi.org/10.1016/j.aninu.2015.12.006

Jingliang M, Yifan H, Jianchang L. 2008. Effect of baogan jiedu decoction on activities of protease and amylase in digestive organs of eel, Anguilla anguilla, with experimental intoxication induced by copper. Chinese Agricultural Science Bulletin. Fuzhou: Fujian Agriculture and Forestry University.

Klahan R, Areechon N, Yoonpundh R, Engkagul A. 2009. Characterization and activity of digestive enzymes in different sizes of Nile Tilapia (Oreochromis niloticus L.). Kasetsart Journal (Nat. Sci.). 43:143-153.

Kužir S, Gjurčević E, Nejedli S, Baždarić B, Kozarić Z. 2012. Morphological and histochemical study of intestine in wild and reared European Eel (Anguilla anguilla L.). Fish Physiology and Biochemistry. 38(3):625–633. https://doi.org/10.1007/s10695-011-9543-7

Lin JC, Wang GL, Min Z. 2015. Purification of protease from intestine of Anguilla japonica and its enzymatic characteristics. [Research article]. College of Environment & Biological Engineering, Putian University.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem. 193:265–275.

Lundstedt LM, Melo JFB, Morales G, 2004. Digestive enzyme and metabolic profile Pseudoplatystoma corruscans (Teleostei : Siluriformes) in response to diet compositian. Comparative Biochemistry and Physiology - Part B. 137(3):331-333. https://doi.org/10.1016/j.cbpc.2003.12.003

Marzuqi M, Anjusary DN. 2013. Kecernaan nutrien pakan dengan kadar protein dan lemak berbeda pada juvenil ikan kerapu pasir (Epinephelus corallicola). Jurnal Ilmu dan Teknologi Kelautan Tropis. 5(2):311-323.

Mazurais D, Kjørsvik E, World PA, Politis SN, Cahu C, Tomkiewicz J, Zambonino IJ. 2013. Biochemical, histological and molecular study of digestive tract development in European eel larvae (Anguilla anguilla) prior to exogenous feeding. Poster session presented at Aquaculture Europe 13. Trondheim, Norway.

Mulyani I, Affandi R, Iswantini D. 2016. Identification of digestive enzyme of Anguilla bicolor bicolor during seed eel phase in controlled container. IOSR Journal of Pharmacy. 6(7):6-11. https://doi.org/10.9790/3013-06720611

Murashita K, Yamamoto T, Nagao J, Furuita H, Awaji M, Tanaka H. 2013. Partial characterization and ontogenetic development of pancreatic digestive enzymes in Japanese eel Anguilla japonica larvae. Fish Physiology and Biochemistry. 39(4):895–905. https://doi.org/10.1007/s10695-012-9749-3

Murtini, S. 2015. Studi makanan alami dan perkembangan anatomi saluran pencernaan ikan sidat (Anguilla bicolor bicolor McClelland 1844) dari muara sungai Cimandiri Pelabuhan Ratu Jawa Barat. [Tesis]. Bogor: Sekolah Pascasarjana IPB.

Novita W, Arief K, Nisa FC, Murdiyatmo U. 2006. Karakterisasi parsial ekstrak kasar enzim protease dari Bacillus amyloliquefaciens NRRL B-14369. Jurnal Teknologi Pertanian. 7(2):96-105.

Pe-a E, Hernández C, González CA, Castro LI, Cruz AP, Hardy RW. 2015. Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus). Latin American Journal of Aquatic Research. 43(4):641-650.

Pujante IM, Lopez MD, Mancera JM, Moyano FJ. 2016. Characterization of digestive enzymes protease and alpha-amylase activities in the thick-lipped grey mullet (Chelon labrosus, Risso 1827). Aquaculture Research. 48(2):367–376. https://doi.org/10.1111/are.13038

Sankar HHS, Jose J, Varadarajan R, Bhanub SV, Joy S, Philip B. 2014. Functional zonation of different digestive enzymes in Etroplus suratensis and Oreochromis mossambicus. International Journal of Scientific and Research Publications. 4(5):1-10.

Saryono. 2011. Biokimia Enzim. Yogyakarta: Nuha Medika.

Sasono AD. 2001. Kebiasaan makan ikan sidat (Anguilla bicolor) di desa Citepus, kecamatan Pelabuhan Ratu dan desa Cimaja, kecamatan Cisolok, kabupaten Sukabumi, Jawa Barat. Skripsi. Bogor: Institut Pertanian Bogor.

Savona B, Tramati C, Mazzola A. 2011. Digestive enzymes in larvae and juveniles of farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777). The Open Marine Biology Journal. 5(1):47-57. https://doi.org/10.2174/1874450801105010047

Setijanto, Sulistyo I, Budianto E. 2014. Penentuan waktu pengambilan benih dan diet ikan sidat (Anguilla bicolor McClelland) di sungai Serayu. Omni-Akuatika. 13(19):46-52.

Shiraishi H, Crook V. 2015. Eel market dynamics: an analysis of Anguilla production, trade and consumption in East Asia. TRAFFIC. Tokyo, Japan.

Suryanti. 2002. Perkembangan aktivitas enzim pencernaan dan hubungannya dengan kemampuan pemanfaatan pakan buatan pada ikan Baung (Mystus nemurus C.V.). [Tesis]. Bogor: Program Pascasarjana IPB.

Susilo U, Yuwono E, Rachmawati FN, Priyanto S, Hana. 2015. Karakteristik enzim digesti, protease dan amilase, ikan Gurami (Osphronemus gouramy Lac.) pada fase pertumbuhan. Biosfera. 32(2):134-142. https://doi.org/10.20884/1.mib.2015.32.2.305

Tesch FW. 2003. The Eel; Biology and Management of Anguillia Eels. 3rd ed. Oxford, UK: Blackwell Publishing.

Wang, K. 2007. Effects of different temperatures and pH on the protease and amylase activities in the digestive tract of Anguilla rostrata. [Research article] . Fujian: Fisheries College & Institute of Aquaculture Biotechnology, Jimei University.

Xiong DM, Xie CX, Zhang HJ, Liu HP. 2011. Digestive enzymes along digestive tract of a carnivorous fish Glyptosternum maculatum (Sisoridae, Siluriformes). Journal of Animal Physiology and Animal Nutrition. 95(1):56–64. https://doi.org/10.1111/j.1439-0396.2009.00984.x

Yandes Z, Affandi R, Mokogintaz I. 2003. Pengaruh pemberian selulosa dalam pakan terhadap kondisi biologis benih ikan Gurami (Osphronemus gouramy). Jurnal lktiologi Indonesia. 3(1):27-33.


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