KETERSERAPAN SENYAWA AKTIF TERPILIH EKSTRAK TEPUNG TEMPE KEDELAI HITAM dan UBI JALAR UNGU pada TIKUS MODEL DIABETES MELLITUS TIPE II

Syahron Mahbub Balada (1)


(1) Universitas Negeri Malang


Abstract


Black soybeans and sweet potatoes contain antioxidants that can inhibit cell damage caused by free radicals. The aim of this study was to determine the absorption of selected active compounds (Isoflavones and Anthocyanins) in type II diabetes (DMT2) rat models. Wistar male rats were used as DMT2 models treated with high fat diet (HFD) and Streptozotosin (STZ) injections until the blood glucose levels reached 160 mg/dl. The experiment was carried out by giving extract of fermented black soybean, which is locally called Tempe, and sweet potato for 28 days on 9 Wistar rats. Rats models were divided into 3 treatment groups. Group I was given with the extract of fermented black soybean flour, group II was given with sweet potato extract, and group III was given with the extract of both fermented black soybean and sweet potato extract with 2:2 ratio. The sample of serum, fecal, and mouse urine were analyzed in the laboratory test by the method of High Pressure Liquid Chromatography (HPLC). The result showed that the absorption of Isoflavones and Anthocyanins extracted from fermented black soybeans and sweet potatoes in type II diabetes rat models were high (99,9%). Therefore, fermented black soybeans and sweet potatoes extract can be used as alternative antidiabetic remedy.

References


Badan Pusat Statistik. 2012. Ubi Jalar Ungu. Jakarta

Badan Standarisasi Nasional. 2012. Tempe. Jakarta

Balitkabi. 2012. Kedelai Hitam: Varietas, Kandungan Gizi dan Prospek Bahan Baku Industri. Jakarta

Chaiyasut, C. W., Kusirisin, N., Lailerd, P., Lerttrakarnnon, M., Suttajit, and Srichairatanakool. 2011. Effects of phenolic compounds of fermented Thai indigenous plants on oxidative stress in streptozotocin-induced diabetic rats). Evidance-Based Complementary and Alternative Medicine Journal, 2011 (3): 1-10

Cheng, J. R., Liu, X. M., Chen, Z. Y., Zhang, Y. S., Zhang, Y. H. 2016. Mulberry anthocyanin biotransformation by intestinal probiotics. Journal Food Chemistry, 32 (7): 1-19.

Djide, M. N., & Permana, A. D. (2014). Ekstraksi Isoflavon Kedelai dan Penentuan Kadarnya Secara Ultra Fast Liquid Chromatography ( UFLC ) Soybean Isoflavones Extraction and Analysis Their Concentration by Ultra Fast Liquid Chromatography. Jurnal Sainsmat, III (2):130-134, ISSN 2086-6755.

Ginting, E., Utomo, J., Yulifianti, R., Jusuf, M. 2011. Potensi Ubijalar Ungu sebagai Pangan Fungsional. Iptek Tanaman Pangan. 1 (6): 116-138.

Hambali, M., Mayasari, F., Noermansyah, F. 2014. Ekstraksi Antosianin Dari Ubi Jalar Dengan Variasi Konsentrasi Solven, dan Lama Waktu Ekstraksi. Teknik Kimia. 2 (20): 25-34.

Hwang, Y. P., Choi, J. H., Han, E. H., Kim, H. G., Wee, J., Jung, K. O., Jung, K H., Kwon, K. I., Jeong, T. C., Chung, Y. C., and Jeong, H. G. 2011. Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphate–activated protein kinase in human HepG2 cells and obese mice. Nutrition Research. 31(12): 896–906.

Krisna, A., Ika, W., & Wardani, R. (2014). Eksplorasi Potensi Kedelai Hitam Untuk Produksi Minuman Fungsional Sebagai Upaya Meningkatkan Kesehatan Masyarakat. Jurnal Pangan Dan Agroindustri. 2(4), 58–67.

Kwon, D. Y., Daily, J. W., Kim, H. J., Park, S. 2009. Antidiabetic Effects of Fermented Soybean Products on Type 2 Diabetes. Nutrition research journal. 20 (3): 1-13.

Kwon, D. Y., Nyakudya, E., Jeong, Y. S. 2014. Fermentation: Food Products. Encylopedia of Agriculture and Food Systems. 3 (10): 113-123.

Liang, L., Wu, X., Zhao, J., Li., F., Zou, Y., Mao, G., & Yang, L. 2012. In Vitro Bioaccesbility and Antioxidant Activity of Anthocyanins from Mulberry (Morus artropurpurea Roxb.) following simulated gastro-intestinal digestion. Food Rresearch International. 46(1): 76-82

Lipovac, M., Pfitscher, A., Hobiger, S., Laschitz, T., Imhof, M., Chedraui, P., & Jungbauer, A. (2015). Red clover isoflavone metabolite bioavailability is decreased after fructooligosaccharide supplementation. Fitoterapia. 10(5), 93–101.

Miura, A., Sugiyama, C., Sakakibara, H., & Simoi, K. (2016). Journal of Nutrition & Intermediary Metabolism Bioavailability of iso fl avones from soy products in equol producers and non-producers in Japanese women. Journal of Nutrition & Intermediary Metabolism, 6(8), 41–47.

Mueller, N.T., Odegard, A. O., Gross, M. D., Koh, W. P., Yu, M. C., Yuan, J. M., Pereira, M. A. 2012. Soy intake and risk of type 2 diabetes mellitus in Chinese Singaporeans: Soy intake and risk of type 2 diabetes. Eur J Nutr 51 (8): 1033-1040.

Sakuraba, H., Mizukami, H., Yagihashi, N., Wada, R., Hanyu, C., Yagihashi, S. 2002. Reduced beta cell mass and expression of oxidative stress-related DNA damage in the islets of Japanese Type II diabetic patients. Diabetologia Journal; 45: 85-96.

Sanjukta, S., & Rai, A. K. (2016). Trends in Food Science & Technology Production of bioactive peptides during soybean fermentation and their potential health bene fi ts. Trends in Food Science & Technology, 50, 1–10. https://doi.org/10.1016/j.tifs.2016.01.010

Setiawati, A. et al. 2014. Analisis Kuantitatif Isoflavon Tempe Secara Cepat dan Sederhana Menggunakan Metode Kromatogrofi Lapis Tipis-Densitometri. Jurnal Farmasi Sains Dan Komunitas, 1 (11), 13-17.

Shim, J. Y., Kim, Y. J., Lee, H. S. 2006. Effect of soybean isoflavone extract on the plasma lipid profiles and antioxidant enzyme activity in streptozotocin-induced diabetic rats. Nutrition research and practice journals, 2 (4): 218-226.

Suda, I., Oki, T., Masuda, M., Kobayashi, M., Nishiba, Y., Furuta, S. 2003. Physiological Functionality of Purple-Fleshed Sweet Potatoes Containing Anthocyanins and Their Utilization in Foods. JARQ, 37 (3): 167-173.

Suhartatik, N., Karyantina, M., Cahyanto, M. N., Raharjo, S., dan Rahayu, E. S. 2014. Karakteristik Fermentatif Medium deMann Rogosa Sharpe (MRS) Antosianin Beras Ketan Hitam (Oryza sativa var. glutinosa) Menggunakan Pediococcus pentosaceus N11.16. Agritech. 34 (3): 291-297.

Vuong,T., Benhaddou-Andaloussi. A., Brault, A., Harbilas, A. Martineau, L. c., Valleran, D., Ramassamy, C., Matar, C., Haddad, P. 2009. Antiobesity and antidiabetic effects of biotransformed blueberry juice in KKA(y) mice. International Journal of Obesity, 33 (2009): 1166-1173.

Yudiono, Kukuk. 2011. Ekstraksi Antosianin Dari Ubi Jalar Ungu (Ipomea batatas cv. AYUMURASAKI) Dengan Teknik Ekstraksi Subcritical Water. Jurnal Teknologi Pangan, 1 (2):25-32.


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