Purwo Raharjo (1) , Windiriani Lestari (2) , Gratiana Ekaningsih Wijayanti (3)

(1) Universitas Jenderal Soedirman
(2) Universitas Jenderal Soedirman
(3) Universitas Jenderal Soedirman


Heavy metals can generally act as EDCs. Likewise chromium hexavalent may serve as a Endocrine Disrupter, especially the fish reproductive system. This study aims to determine the effect of chromium hexavalent on estradiol content, ovarian development and liver of nilem female (Osteochilus hasselti C.V.), the researchers also want to see the dose of chromium hexavalent most influential on estradiol levels, ovarian development, and liver of nilem fish (Osteochilus hasselti C.V.) as well as the interaction between reproduction parameters with water quality of maintenance media of nilem fish (Osteochilus hasselti C.V.).

This study uses the method of "quantitative experimental". The experimental design was Completely Randomized Design with treatment of chromium hexavalent dose of 0, 0.009, 0.018, 0.027 and 0.036 mg/L performed in 5 replications at 3 stages, ie 1st, 2nd, and 3th months post-spawning maintenance. At 1st month of maintenance, chromium hexavalent did not significantly affect the reproductive parameters of GSI (Gonadosomatic Index), fecundity, HSI and estradiol. The chromium hexavalent had no significant effect on GSI, fecundity and estradiol and significantly affected HSI at 2nd months of maintenance. Chromium hexavalent significantly affected GSI, fecundity, HSI and estradiol in a 3th month maintenance trial.


Banu, S. K., J. B. Samuel, J. A. Arosh, R. C. Burghardt, dan M. M. Aruldhas. 2008. Lactational Exposure to Hexavalent Chromium Delays Puberty by Impairing Ovarian Development, Steroidogenesis and Pituitary Hormone Synthesis in Developing Wistar Rats. Toxicol Appl Pharmacol., 232: 180–189.

Byrne, C., S. D. Divekar, G. B. Storchan, D. A. Parodi dan M. B. Martin. 2013. Metals and Breast Cancer. Journal of Mammary Gland Biology and Neoplasia, 18(1): 63-73.

Cambell, B., J. Dickey, B. Deckman, G. Young, A. Pierce, H. Fukada dan P. Swanson. 2006. Previtellogenic Oocyte Growth In Salmon: Relationships Among Body Growth, Plasma Insulin-Like Growth Factor 1 Estradiol-17β, Follicle-Stimulating Hormone and Expression of Ovarian Genes For Insulin-Like Growth Steroidegenic-Acute Regulatoryprotein and Receptors for Gonadotropins, Growth Hormone and Somatolactin. Biology of Reproduction, 75: 34-44.

Cerda, J., B. G. Caiman G. J., Lafleur Jr. dan S. Limesand. 1996. Pattern of Vitellogenesis and Ovarian Folicular Cycle of Fundulus Heteroclitus. General and Comparative Endocrinology, 103: 24 - 35.

Devlin, R. H dan Y. Nagahama. 2002. Sex Determination and Sex Differentiation in Fish: an Overview of Genetic, Physiological and Environmental Influences. Aquaculture, 208: 191-364.

Effendie, M. I. 1979. Metoda Biologi Perikanan. Yayasan Dewi Sri, Bogor.

Fromm, P. O. 1980. A Review of Some Physiological and Toxicological Responses of Freshwater Fish to Acid Stress. Environmental Biology of Fishes, 5(1): 79-93.

Gaikwad, M. V., V. R. More, S. M. Shingare, D. K. Hiwarale dan Y. K. Khillare. 2009. Study on Gonadosomatic and Fecundity Relationship in air-Breathing Fish Channa gachua (Ham.) From Godavari Near Aurangabad. African Journal of Basic & Applied Science, 1(5-6): 93-95.

Ghufran, M. dan K. Kordi. 2010. Buku Pintar Pemeliharaan 14 Ikan Air Tawar Ekonomis di Keramba Jaring Apung. Lily Publisher. Yogyakarta.

Hoar WS, Randall DJ, Donaldson EM. 1983. Fish Physiology Volume

IX Reproduction Part B Behavior and Fertility Control. New

York: Academic Press, Inc

Hoar WS, Randall DJ, Donaldson EM. 1983. Fish Physiology Volume

IX Reproduction Part B Behavior and Fertility Control. New

York: Academic Press, Inc

Hoar WS, Randall DJ, Donaldson EM. 1983. Fish Physiology Volume IX Reproduction Part B Behavior and Fertility Control. New York: Academic Press, Inc.

Huuskonen, S. dan P. Lindström-Seppä. 1995. Hepatic Cytochrome P4501A and Other Biotransformation Activities in Perch (Perca fluviatilis): The Effects of Unbleached Pulp Mill Effluents. Aquatic Toxicology, 31(1): 27–41.

Kumari, K., K. Ankur dan D. Swati. 2014. The Applicability of Oxidative Stress Biomarkers in Assessing Chromium Induced Toxicity in the Fish Labeo rohita. BioMed Research International, 2014: 11 hal.

Kusrini, E., A. Priyadi, G. S. Wibawa, dan I. Insan. 2010. Pengaruh pH Terhadap Perkembangan Gonad Ikan Rainbow Sawiat (Melanotaenia sp.). Balai Riset Budidaya Ikan Hias, Depok.

Miranda, L. A., C. A. Strussmann dan G. M. Somoza. 2009. Effects of Light and Temperature Conditions on the Expression of GnRH and GtH Genes and Levels of Plasma Steroids in Odontesthes bonariensis Females. Fish Physiology and Biochemistry, 35: 101 108.

Mishra, A. K dan B. Mohanty. 2012. Effect of Sublethal Hexavalent Chromium Exposure on the Pituitary-ovarian Axis of a Teleost, Channa punctatus (Bloch). Environ Toxicol, 27: 415–422.

Mount, D. I. 1973. Chronic Effect of Low pH on Fathead Minnow Survival, Growth and Reproduction. Water Research Pergamon Press, 7: 987-993.

Moyle, P. B. dan J. C. Jr. Joseph. 2000. Fishes: An Introduction to Ichthyology 4th Ed. Uppersaddle River, NJ: Prentice Hal. 612 hal.

Pait, A. S. dan O. N. Judd. 2002. Endocrine Disruption in Fish: An Assessment of Recent Research and Results. Silver Spring, MD, NOAA/National Ocean Service/National Centers for Coastal Ocean Science, (NOAA Technical Memorandum NOS NCCOS CCMA, 149), 55 hal.

Prayogo, N. A., G. E. Wijayanti, Murwantoko, M. Kawaichi dan P. Astuti. 2012. Effect of Photoperiods on Melatonin Levels, the Expression of cGnRH-II and sGnRH Genes and Estradiols Level in Hard-Lipped Barb (Osteochilus hasselti C.V.). Global Veterinaria, 8(6): 591-597.

Rochmatin, S. Y., A. Solichin dan S.W. Saputra. 2014. Aspek Pertumbuhan Dan Reproduksi Ikan Nilem (Osteochilus hasselti) Di Perairan Rawa Pening Kecamatan Tuntang Kabupaten Semarang. Diponegoro Journal Of Maquares, 3(3): 153-159.

Sadekarpawar, S. dan P. Pragna. 2013. Gonadosomatic and Hepatosomatic Indices of Freshwater Fish Oreochromis mossambicus in Response to a Plant Nutrient. World Journal of Zoology, 8(1): 110-118.

Sari, A. M., R. Fida. dan F. Herlina. 2014. Pengaruh Cekaman Kromium pada Limbah Cair Batik terhadap Pertumbuhan Eichornia crassipes dan Salvinia molesta. LenteraBio, 3(1): 67–71.

Sasongko, D. P. dan P. T. Wildan. 2010. Identifikasi Unsur dan Kadar Logam Berat pada Limbah Pewarna Batik dengan Metode Analisis Pengaktifan Neutron. Jurnal Ilmu Pengetahuan dan Teknologi TELAAH, 27: 22-27.

Sharifuddin, B. O. A. 2010. Aspek reproduksi ikan nilem, Osteochilus vittatus (Valenciennes, 1842) di Danau Sidenreng, Sulawesi Selatan. Jurnal Iktiologi Indonesia, 10(2):111-122.

Singh, S. 2007. Impact of an Insecticide Rogor On Ovary of Channa Punctatus (Bloch). Nature Environment and Pollution Technology, 6(3): 471-475.

Susanto, H. 2001. Budidaya Ikan di Pekarangan. Penebar Swadaya. Jakarta.

Velma, V dan P. B. Tchounwou. 2011. Hexavalent Chromium-Induced Multiple Biomarker Responses in Liver and Kidney of Goldfish, Carassius auratus. Environ Toxicol, 26(6): 649–656.

Wallace, R. A. dan K. Selman. 1981. Cellular and Dynamic Aspects of Oocyte Growth in Teleosts. Am. Zool., 21: 325-343.

Wijayanti, G. E., Soeminto dan S. B. I. Simanjuntak. 2009. Profil hormon Reproduksi dan Gemetogenesis pada Gurame (Osphronemus gouramy Lac.) Betina. Jurnal Akuakultur Indonesia, 8(1): 77-89.

Wu, R. S. S. 2009. Chapter 3: Effects of Hypoxia on Fish Reproduction and Development. Fish Physiology, 27: 79-141.

Zhou, B. S., R. S. S. Wu, D. J. Rendall dan P. K. S. Lam. 2001. Bioenergetics and RNA/ DNA Ratios in the Common Carp (Cyprinus carpio) Under Hypoxia. J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol., 171: 49–57.

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